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Am. J. Trop. Med. Hyg., 72(3), 2005, pp. 278-283
Copyright © 2005 by The American Society of Tropical Medicine and Hygiene

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APPLICATION OF SYNTHETIC 8-KD AND RECOMBINANT GP50 ANTIGENS IN THE DIAGNOSIS OF NEUROCYSTICERCOSIS BY ENZYME-LINKED IMMUNOSORBENT ASSAY

EDNEIA C. BUENO, CHRISTINA M. SCHEEL, ADELAIDE J. VAZ, LUIS R. MACHADO, JOSÉ A. LIVRAMENTO, OSVALDO M. TAKAYANAGUI, VICTOR C. W. TSANG, AND KATHY HANCOCK
Faculty of Pharmacy, University of the Vale do Itajaí, Itajaí, Santa Catarina, Brazil; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Faculty of Pharmacy, and Faculty of Medicine, University of São Paulo, São Paulo, Brazil; Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

The gold standard serodiagnostic assay for cysticercosis and neurocysticercosis, diseases caused by the metacestode of Taenia solium, uses lentil lectin-purified glycoprotein (LLGP) in a Western blot assay. We tested two antigens derived from LLGP, synthetic TS18var1 (sTS18var1) and recombinant GP50 antigen (rGP50), in an enzyme-linked immunosorbent assay (ELISA) using serum and cerebrospinal fluid (CSF) samples. The sensitivity for serum and CSF was 94.7% and 100% for rGP50 and 90.4% and 90.2% for sTS18var1, respectively. The specificity for serum and CSF samples was 93.8% and 100% for rGP50 and 90.3% and 98.0% for sTS18var1, respectively. The use of these antigens individually or combined as a diagnostic antigen cocktail eliminates the need for purification of antigens from parasite material and offers the advantage of using a simple and quantitative ELISA format.

Received July 9, 2004. Accepted for publication October 9, 2004.

Acknowledgments: We thank Juliana B. Pretto for collecting sera from healthy individuals, and Andreia Gomes for collecting the samples from neurocysticercosis patients. We also thank Melinda L. Yushak for her work in organizing the CDC collection of defined parasitic infection sera, and Azra Khan for her advice regarding the rGP50 assay.

Financial support: Support for sample shipment was provided by FAPESP 2002/12061-0.

Authors’addresses: Edneia C. Bueno, Curso de Farmácia, Universidade do Vale do Itajaí, Rua Uruguai, 458, Bloco 17, Sala 205, Itajaí, SC, 88.302-202, Brazil. Christina M. Scheel, Victor C. W. Tsang, and Kathy Hancock, Division of Parasitic Diseases, Centers for Disease Control and Prevention, Building 23, Mailstop F-13, 4770 Buford Highway, Atlanta, GA, 30341. Adelaide J. Vaz, Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 580, Bloco 17, São Paulo, SP, 05.508-900, Brazil. Luis R. Machado and José A. Livramento, Centro de Investigações em Neurologia, Faculdade de Medicina, Universidade de São Paulo, Av. Dr. Arnaldo, 455, 01.246-903, São Paulo, SP, Brasil. Osvaldo M. Takayanagui, Departamento de Neuropsiquiatria e Psicologia Médica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto, SP, 14.049-900, Brazil.

Reprint requests: Edneia C. Bueno, Curso de Farmácia, Universidade do Vale do Itajaí, Rua Uruguai, 458, Bloco 17, Sala 205, Itajaí, SC, 88.302-202, Brazil, Telephone: 55-47-341-7833, Fax: 55-47-341-7501, E-mail: ecbueno{at}ccs.univali.br.




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Copyright © 2005 by the American Society of Tropical Medicine and Hygiene.