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To evaluate the prevalence of toxocariasis in children in Jaboatão dos Guararapes, Pernambuco in northeastern Brazil, 215 serum samples were examined by an enzyme-linked immunosorbent assay (ELISA) using a recombinant Toxocara canis antigen. In the ELISA, 26 (12.1%) of 215 subjects were positive. In a dot-blot assay using 53 of 215 serum samples, the diagnostic results correlated with those obtained by the ELISA. Moreover, it has been confirmed that the recombinant T. canis antigen was highly specific for toxocariasis by ELISA using serum samples positive for antibody to Ascaris lumbricoides. Considering the specificity of the recombinant antigen to toxocariasis, the ELISA or dot-blot assay using the recombinant T. canis antigen is recommended in tropical and sub-tropical regions where various parasitic infections are commonly endemic.
Received June 7, 2004. Accepted for publication July 7, 2004.
Financial support: This work was supported in part by the Japan International Cooperation Agency as a part of an international cooperative project for the Laboratório de Imunopatología Keizo Asami, Universidade Federal de Pernambuco, Brazil.
Authors addresses: Raquel de Andrade Lima Coêlho and Luiz Bezerra de Carvalho, Jr., Departamento de Bioquímica, Laboratório de Imunopatología Keizo Asami, Universidade Federal de Pernambuco, Campus Universitário, 50670-901 Recife, Pernambuco, Brazil. Emília Pessoa Perez, Departamento de Parasitología, Centro de Pesquisas Aggeu Magalhães-FIOCRUZ, Cidade Universitária, 50670-901 Recife, Pernambuco, Brazil. Kunioki Araki, Section of Environmental Parasitology, Tokyo Medical and Dental University, Graduate School, Tokyo 113-8519, Japan. Tsutomu Takeuchi, Department of Tropical Medicine and Parasitology, Keio University School of Medicine, Tokyo 160-8582, Japan. Akira Ito and Hiroshi Yamasaki, Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan. Takashi Aoki, Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, Tokyo 113-8421, Japan.
Reprint requests: Hiroshi Yamasaki, Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2-1-1-1, Asahikawa 078-8510, Japan. Telephone: 81-166-68-2421. Fax: 81-166-68-2429. E-mail: hyamasak{at}asahikawa-med.ac.jp.
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