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DETECTION OF TOXOPLASMA GONDII DNA AND SPECIFIC ANTIBODIES IN HIGH-RISK PREGNANT WOMEN

Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission to the fetus. This study was conducted to test the utility of a polymerase chain reaction (PCR) assay to detect recent infections with Toxoplasma in pregnant women. One hundred forty-eight women with high-risk pregnancies who had abnormal pregnancy outcomes (cases) and 100 with normal pregnancies (controls) were tested for the presence of Toxoplasma DNA in their blood by a nested PCR and specific antibodies to Toxoplasma by an enzyme-linked immunosorbent assay. The IgG results of the cases differed significantly from those of the controls (54% and 12%, respectively; P < 0.02). Four (2.7%) of the cases were IgM positive, but none of the controls were positive. Detection of Toxoplasma DNA in 20 (8.1%) of the IgG-positive cases suggests a recent infection. The risk factors associated with the infection were eating raw meat and contact with soil. The diagnostic serology of recent infection in early pregnancy could be confirmed by a positive Toxoplasma-specific PCR result in blood samples collected in the first half of pregnancy, even in the presence of serologic results difficult to interpret due to the lack of sequential follow-up during pregnancy.

Received May 31, 2004. Accepted for publication June 28, 2004.

Financial support: This work was supported by Jordan University of Science and Technology (grant no. 49/99).

Authors’ addresses: Laila Nimri and Layla Elkhatib, Department of Medical Laboratory Sciences, Jordan University of Science and Technology, PO Box 3030, Irbid 22110, Jordan. Telephone: 962-2-720-100, Fax: 962-2-709-5070, E-mail: nimri{at}just.edu.jo. Hervé Pelloux, Service de Parasitologie-Mycologie, Centre Hospitalier et Universitaire, Michallon, BP 217, 38043 Grenoble, France, E-mail: HPelloux{at}chugrenoble.fr.

Reprint requests: Department of Medical Laboratory Sciences, Jordan University of Science and Technology, PO Box 3030, Irbid 22110, Jordan.

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