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Am. J. Trop. Med. Hyg., 71(4), 2004, pp. 400-402
Copyright © 2004 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT: FLUORESCENT LEISHMANIA: APPLICATION TO ANTI-LEISHMANIAL DRUG TESTING

NEELOO SINGH AND ANURADHA DUBE
Division of Biochemistry and Division of Parasitology, Central Drug Research Institute, Lucknow, India

 

ABSTRACT

Classic techniques for detecting the susceptibility of Leishmania to different drugs are time-consuming, laborious, and require the use of macrophages. The use of flow cytometry for monitoring Leishmania susceptibility to drugs is beginning to be implemented. Using green fluorescent protein (GFP), we have improved and simplified the screening procedure. We introduced a GFP marker into field strains of Leishmania causing kala-azar (visceral leishmaniasis) and explored the suitability of transgenic L. donovani promastigotes that constitutively express GFP in their cytoplasm as target cells for in vitro screening of anti-leishmanial drugs.

Received July 29, 2003. Accepted for publication November 9, 2003.

Acknowledgment: We are indebted to Stephen Beverley (Washington University, St. Louis, MO) for plasmid pXG-GFP.

Authors’ addresses: Corresponding author: Neeloo Singh, Division of Biochemistry, Central Drug Research Institute, Lucknow, India, Telephone: 91-522-212-41118, Fax: 91-522-222-3405, E-mail: neeloo888{at}yahoo.com. Anuradha Dube, Division of Parasitology, Central Drug Research Institute, Lucknow, India.






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