| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Dengue is a serious cause of morbidity and mortality in tropical areas worldwide. We cloned and expressed recombinant polypeptides spanning the entire genome of a Brazilian dengue virus type 2 (DENV-2) strain in contiguous segments to generate antigens for dengue diagnosis and evaluation of the human humoral immune response. When analyzed by Western blot and an enzyme-linked immunosorbent assay (ELISA) using human sera, the most reactive polypeptide (pD2-3(E)) was located in the N-terminal portion of the envelope protein. The sensitivity of an IgG-ELISA using pD2-3(E) versus mouse brain antigen was 100% with convalescent sera and 79% with acute sera, with a specificity of 100%. Sera from patients infected with other DENV serotypes recognized pD2-3(E) equally well, whereas sera positive for yellow fever, rubella, and measles showed little or no reactivity. Using this novel approach, we identified a candidate antigen to facilitate diagnosis of DENV infections and observed a surprising variability in antibody patterns in the clinical response to DENV infections.
Received October 3, 2003. Accepted for publication February 7, 2004.
Acknowledgments: We are grateful to Brendan Flannery for advice regarding protein expression and purification.
Financial support: This research was supported by Fogarty International Center grant TW 00905. Marize Pereira Miagostovich, Rita Maria Ribeiro Noguiera, and Hermann Gonçalves Schatzmayr were fellows of CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico grant 40.0164/98-1).
Authors’ addresses: Flavia Barreto dos Santos, Marize Pereira Miagostovich, Rita Maria Ribeiro Noguiera, and Hermann Gonçalves Schatzmayr, Department of Virology Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil, 4365, Manguinhos, Caixa Postal 926, CEP 21045-900 Rio de Janeiro, Brazil, Telephone: 55-21-2598-4373, Fax: 55-21-2598-4491. Lee W. Riley and Eva Harris, Division of Infectious Diseases, School of Public Health, University of California, 140 Warren Hall, Berkeley, CA 94720-7360, Telephone: 510-642-4845, Fax: 510-642-6350, E-mail: eharris{at}socrates.berkeley.edu.
This article has been cited by other articles:
|
|
 |
|
  T. R. Prestwood, D. M. Prigozhin, K. L. Sharar, R. M. Zellweger, and S. Shresta A Mouse-Passaged Dengue Virus Strain with Reduced Affinity for Heparan Sulfate Causes Severe Disease in Mice by Establishing Increased Systemic Viral Loads J. Virol., September 1, 2008; 82(17): 8411 - 8421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. B. Dos Santos, R. M. R. Nogueira, M. R. Q. Lima, T. S. De Simone, H. G. Schatzmayr, E. M. B. Lemes, E. Harris, and M. P. Miagostovich Recombinant Polypeptide Antigen-Based Immunoglobulin G Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Dengue Clin. Vaccine Immunol., May 1, 2007; 14(5): 641 - 643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shresta, K. L. Sharar, D. M. Prigozhin, P. R. Beatty, and E. Harris Murine Model for Dengue Virus-Induced Lethal Disease with Increased Vascular Permeability. J. Virol., October 1, 2006; 80(20): 10208 - 10217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Videa, M. J. Coloma, F. B. dos Santos, A. Balmaseda, and E. Harris Immunoglobulin M Enzyme-Linked Immunosorbent Assay Using Recombinant Polypeptides for Diagnosis of Dengue Clin. Vaccine Immunol., July 1, 2005; 12(7): 882 - 884. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
