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Am. J. Trop. Med. Hyg., 71(1), 2004, pp. 40-42
Copyright © 2004 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT: POLYMORPHISMS IN THE CHLOROQUINE RESISTANCE TRANSPORTER GENE IN PLASMODIUM FALCIPARUM ISOLATES FROM LOMBOK, INDONESIA

MARIA CECILIA HUAMAN, KAZUMI YOSHINAGA, AAN SURYANATHA, NYOMAN SUARSANA, AND HIROJI KANBARA
Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki, Japan; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru; Meninting Health Center, West Lombok, Indonesia

 

ABSTRACT

The polymorphisms in the Plasmodium falciparum multidrug resistance 1 (pfmdr1) and P. falciparum chloroquine resistance transporter (pfcrt) genes, which are associated with chloroquine resistance, were examined in 48 P. falciparum isolates from uncomplicated malaria patients from the West Lombok District in Indonesia. The point mutation N86Y in pfmdr1 was present in 35.4% of the isolates and mutation K76T in pfcrt was found in all but one of the samples studied. Identified pfcrt haplotypes were mainly identical to the Papua New Guinea type SagtVMNT (42 of 48, 87.5%), and a few isolates had the Southeast Asia type CVIET (5 of 48, 10.4%). Moreover, one P. falciparum isolate harbored the K76N mutation, giving rise to the haplotype CVMNN, which was not previously reported in field isolates. Our findings suggest that chloroquine resistance in this area might have the same origin as in Papua New Guinea.

Received December 16, 2003. Accepted for publication February 4, 2004.

Acknowledgments: We are grateful to the Case Detection and Treatment Team at the Meninting Health Center (West Lombok, Indonesia) for their collaboration in this study.

Financial support: This study was partially supported by the Japanese International Cooperation Agency partnership program, and by grant for International Health Cooperation Research (13-C-5) from the Ministry of Health, Labor and Welfare. Maria Cecilia Huaman is the recipient of the Monbusho scholarship awarded by the Ministry of Education, Science, Sports and Culture of the Government of Japan.

Authors’ addresses: Maria Cecilia Huaman, Institute of Tropical Medicine, Nagasaki University, Sakamoto 1-12-4, Nagasaki 852-8523, Japan and Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru, E-mail: ceci_ huaman{at}yahoo.com. Kazumi Yoshinaga and Hiroji Kanbara, Institute of Tropical Medicine, Nagasaki University, Sakamoto 1-12-4, Nagasaki 852-8523, Japan, Telephone: 81-95-849-7838, Fax: 81-95-849-7805. E-mail: f0512{at}cc.nagasaki-u.ac.jp. Aan Suryanatha and Nyoman Suarsana, Meninting Health Center, West Lombok, Indonesia.






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