AJTMH HINARI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 70(6), 2004, pp. 597-603
Copyright © 2004 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (20)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by PIDIYAR, V. J.
Right arrow Articles by SHOUCHE, Y. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by PIDIYAR, V. J.
Right arrow Articles by SHOUCHE, Y. S.
Related Collections
Right arrow Vector Biology

STUDIES ON CULTURED AND UNCULTURED MICROBIOTA OF WILD CULEX QUINQUEFASCIATUS MOSQUITO MIDGUT BASED ON 16S RIBOSOMAL RNA GENE ANALYSIS

VYANKATESH J. PIDIYAR*, KAMLESH JANGID*, MILIND S. PATOLE, AND YOGESH S. SHOUCHE
Molecular Biology Unit, National Centre for Cell Science, University of Pune, Pune, India

To describe the midgut microbial diversity and the candidate bacteria for the genetic manipulation for the generation of transgenic mosquitoes refractory to transmission of diseases, the microbiota of wild Culex quinquefasciatus mosquito midgut was studied using a conventional culture technique and analysis of a 16S ribosomal RNA (rRNA) gene sequence library. The culturable microbiota was identified as Acinetobacter junii, Ac. calcoaceticus, Aeromonas culicicola, Bacillus thuringiensis, Microbacterium oxydans, Pantoea agglomerans, Pseudomonas aeruginosa, Staphylococcus epidermidis, Stenotrophomonas maltophila and an unidentified bacterium from the host Drosophila paulistorium. The 16S rRNA gene library was composed of 46% unidentified and uncultured bacteria, 41% Acinetobacter spp., and 13% Lactococcus spp. The coverage calculated for the 150 clones was 83.3%. Thus, the probability of the next cloned sequence falling in a novel operational taxonomic unit (not yet observed) was 16.7%. The majority of the cultured isolates and the 16S rRNA gene library clones belonged to the {gamma}-proteobacteria class. Most of the bacteria have been previously reported to inhabit the midgut of different mosquito species. Therefore, the results of this study indicate that different mosquito species harbor common representatives of the microbiota that may be the potential candidates for genetic manipulation to control the disease transmission capabilities of the host.

Received October 6, 2003. Accepted for publication November 3, 2003.

* These authors contributed equally to this report.

Authors’ address: Vyankatesh J. Pidiyar, Kamlesh Jangid, Milind S. Patole, and Yogesh S. Shouche, Molecular Biology Unit, National Centre for Cell Science, Pune University Campus, Ganeshkhind, Pune-411007, Maharashtra, India, Telephone: 91-20-569-0922, Fax: 91-20-569-2259, E-mails: vpidiyar{at}hotmail.com, jangidk{at}nccs.res.in, milindpatole{at}hotmail.com, and yogesh{at}nccs.res.in.




This article has been cited by other articles:


Home page
 Int. J. Syst. Evol. Microbiol.Home page
S. Shivaji, P. Chaturvedi, Z. Begum, P. K. Pindi, R. Manorama, D. A. Padmanaban, Y. S. Shouche, S. Pawar, P. Vaishampayan, C. B. S. Dutt, et al.
Janibacter hoylei sp. nov., Bacillus isronensis sp. nov. and Bacillus aryabhattai sp. nov., isolated from cryotubes used for collecting air from the upper atmosphere
Int J Syst Evol Microbiol, December 1, 2009; 59(12): 2977 - 2986.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
K. Zouache, D. Voronin, V. Tran-Van, and P. Mavingui
Composition of Bacterial Communities Associated with Natural and Laboratory Populations of Asobara tabida Infected with Wolbachia
Appl. Envir. Microbiol., June 1, 2009; 75(11): 3755 - 3764.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
V. Corby-Harris, A. C. Pontaroli, L. J. Shimkets, J. L. Bennetzen, K. E. Habel, and D. E. L. Promislow
Geographical Distribution and Diversity of Bacteria Associated with Natural Populations of Drosophila melanogaster
Appl. Envir. Microbiol., June 1, 2007; 73(11): 3470 - 3479.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Favia, I. Ricci, C. Damiani, N. Raddadi, E. Crotti, M. Marzorati, A. Rizzi, R. Urso, L. Brusetti, S. Borin, et al.
Bacteria of the genus Asaia stably associate with Anopheles stephensi, an Asian malarial mosquito vector
PNAS, May 22, 2007; 104(21): 9047 - 9051.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Enol. Vitic.Home page
W. H. Groenewald, C. A. Van Reenen, S. D. Todorov, M. D. Toit, R. C. Witthuhn, W. H. Holzapfel, and L. M.T. Dicks
Identification of Lactic Acid Bacteria from Vinegar Flies Based on Phenotypic and Genotypic Characteristics
Am. J. Enol. Vitic., December 1, 2006; 57(4): 519 - 525.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
P. L. Worthen, C. J. Gode, and J. Graf
Culture-independent characterization of the digestive-tract microbiota of the medicinal leech reveals a tripartite symbiosis.
Appl. Envir. Microbiol., July 1, 2006; 72(7): 4775 - 4781.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
J. M. Lindh, O. Terenius, and I. Faye
16S rRNA Gene-Based Identification of Midgut Bacteria from Field-Caught Anopheles gambiae Sensu Lato and A. funestus Mosquitoes Reveals New Species Related to Known Insect Symbionts
Appl. Envir. Microbiol., November 1, 2005; 71(11): 7217 - 7223.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the American Society of Tropical Medicine and Hygiene.