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Am. J. Trop. Med. Hyg., 70(3), 2004, pp. 294-297
Copyright © 2004 by The American Society of Tropical Medicine and Hygiene

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A SENSITIVE NEW MICROCULTURE METHOD FOR DIAGNOSIS OF CUTANEOUS LEISHMANIASIS

ADIL M. ALLAHVERDIYEV, SONER UZUN, MALAHAT BAGIROVA, MURAT DURDU, AND HAMDI R. MEMISOGLU
Tropical Diseases Research Centre, and Department of Dermatology, School of Medicine, Cukurova University, Adana, Turkey

A sensitive microcapillary culture method (MCM) was developed for rapid diagnosis of cutaneous leishmaniasis (CL). The MCM is superior to the traditional culture method (TCM) as determined by the smaller inoculum size, the higher sensitivity for detection of promastigotes, and the more rapid time for emergence of promastigotes. With lesion amastigote loads from grade III to 0, the positive rates and the periods for promastigote emergence were 3–4-fold higher and faster with the MCM than with the TCM, e.g., 83–97% positive in 4–7 days versus 20–40% positive in 15–30 days (P = 0.0001). The higher PCO2 and lower PO2 and pH presumably encourage a rapid amastigoteto-promastigote differentiation and/or the survival or growth of the latter. This MCM has the advantage of simplicity, and may be suitable for diagnostic use and for parasite retrieval in many other endemic sites where parasites are known to be difficult to grow.

Received August 1, 2003. Accepted for publication November 13, 2003.

Acknowledgments: We thank K.-P. Chang (University of Health Sciences/Chicago Medical School, Chicago, IL) for reviewing the manuscript.

Authors’ addresses: Adil M. Allahverdiyev and Malahat Bagirova, Tropical Diseases Research Centre, School of Medicine, Cukurova University, 01330, Adana, Turkey, Telephone: 90-322-338-60-60/ 3425, Fax: 90-322-338-64-26, E-mails: yay{at}cu.edu.tr and mmalahat{at}yahoo.com. Soner Uzun, Murat Durdu, and Hamdi R.Memisoglu, Department of Dermatology, School of Medicine, Cukurova University, 01330, Adana, Turkey, E-mail: sonuzun{at}hotmail.com.




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