HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (23) Citing Articles via Google Scholar Google Scholar Articles by GARROS, C. Articles by MANGUIN, S. Search for Related Content PubMed PubMed Citation Articles by GARROS, C. Articles by MANGUIN, S. Related Collections Vector Biology Malaria Medical Entomology

RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD FOR THE IDENTIFICATION OF MAJOR AFRICAN AND ASIAN MALARIA VECTORS WITHIN THE ANOPHELES FUNESTUS AND AN. MINIMUS GROUPS

The African Anopheles funestus and the Asian An. minimus groups are closely related and are probably considered distinct only because of their geographic separation. This study aimed at improving two identification methods based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) already developed for either group. Each PCR-RFLP, either on the internal transcribed spacer 2 (ITS2) for the An. minimus group, and domain 3 (D3) for the An. funestus group, was applied to the other group for the standardization of one identification method applicable on both continents. The ITS2 fragment digested by Bsi ZI showed the highest diagnostic power. This assay allowed the discrimination of at least 13 Anopheles species within the subgenus Cellia from two continents (Africa and Asia), among which are five major malaria vectors. Moreover, digestion of the D₃ with Msp I showed intragenomic variations within An. funestus populations. Two types of D3 copies (M and W) occurred in specimens from southern Africa. The populations from West-Central Africa presented only type W and East-Malagasy populations exhibited type M. Since An. funestus shows a great capacity of adaptation, these molecular variations, along with behavioral and ecologic ones, reinforce the hypothesis of a species complex that will need to be further investigated.

Received May 3, 2003. Accepted for publication November 10, 2003.

Acknowledgments: This study was made possible because of the assistance of a number of people who provided mosquito samples from both continents (subtropical Africa and Southeast Asia). We are especially grateful to D. Fontenille, B. M. Ntomwa, and H. D. Trung for providing us with mosquitoes populations from West-Central Africa, Angola, and Vietnam, respectively.

Financial support: This work was partially supported by a European Community grant (research project ERBIC 18CT970211); a VIH-PAL (HIV/Malaria) 2000 grant from the French Ministry of Research to S. Manguin; the World Health Organization/AFRO for a multi-center project to M. Coosemans; a grant from the UNDP/World Bank/World Health Organization Special Program for Research and Training in Tropical Diseases to L. L. Koekemoer; and a grant from South African Medical Research Council and the National Health Laboratory Service to L. L. Koekemoer.

Authors’ addresses: C. Garros and S. Manguin, Centre of Biology and Management of Populations, Campus de Baillarguet, CS 30016, 34988 Montferrier sur Lez, France, Telephone: 33- 4-99-62-33-28/27, Fax: 33-4-99-62-33-45, E-mails: garros{at}mpl.ird.fr and manguin{at}mpl.ird.fr. L. L. Koekemoer and M. Coetzee, Medical Entomology, Department of Clinical Microbiology and Infectious Diseases, School of Pathology of the South African Institute for Medical Research and the University of the Witwatersand, PO Box 1038, Johannesburg, South Africa, Telephone: 27-11-489-9390, Fax: 27-11-489-9399. L. Kamau and T. S. Awolola, Centre for Biotechnology Research and Development, Kenya Medical Research Institute, PO Box 54840, Nairobi, Kenya. W. Van Bortel and M. Coosemans, Department of Parasitology, Prince Leopold Institute of Tropical Medicine, Nationalestraat 155, B-2000, Antwerp, Belgium.

Reprint requests: C. Garros or S. Manguin, Centre of Biology and Management of Populations, Campus de Baillarguet, CS 30016, 34988 Montferrier sur Lez, France.

This article has been cited by other articles:

				B. L. Spillings, B. D. Brooke, L. L. Koekemoer, J. Chiphwanya, M. Coetzee, and R. H. Hunt A New Species Concealed by Anopheles funestus Giles, a Major Malaria Vector in Africa Am J Trop Med Hyg, September 1, 2009; 81(3): 510 - 515. [Abstract] [Full Text] [PDF]

				M. A. Zapata, A. V. Cienfuegos, O. I. Quiros, M. L. Quinones, S. Luckhart, and M. M. Correa Discrimination of Seven Anopheles Species from San Pedro de Uraba, Antioquia, Colombia, by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Its Sequences Am J Trop Med Hyg, July 1, 2007; 77(1): 67 - 72. [Abstract] [Full Text] [PDF]

				V. PINEDO-CANCINO, P. SHEEN, E. TARAZONA-SANTOS, W. E. OSWALD, C. JERI, A. Y. VITTOR, J. A. PATZ, and R. H. GILMAN LIMITED DIVERSITY OF ANOPHELES DARLINGI IN THE PERUVIAN AMAZON REGION OF IQUITOS. Am J Trop Med Hyg, August 1, 2006; 75(2): 238 - 245. [Abstract] [Full Text] [PDF]

				C. S. Wondji, R. H. Hunt, P. Pignatelli, K. Steen, M. Coetzee, N. Besansky, N. Lobo, F. H. Collins, J. Hemingway, and H. Ranson An Integrated Genetic and Physical Map for the Malaria Vector Anopheles funestus Genetics, December 1, 2005; 171(4): 1779 - 1787. [Abstract] [Full Text] [PDF]