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Am. J. Trop. Med. Hyg., 69(3), 2003, pp. 309-313
Copyright © 2003 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT: QUANTIFICATION OF LEISHMANIAVIRUS RNA IN CLINICAL SAMPLES AND ITS POSSIBLE ROLE IN PATHOGENESIS

MONICA M. OGG, RICARDO CARRION, JR., ANA CRISTINA DE CARVALHO BOTELHO, WILSON MAYRINK, RODRIGO CORREA-OLIVEIRA, AND JEAN L. PATTERSON
Department of Microbiology University of Texas Health Science Center San Antonio, San Antonio, Texas; Department of Virology and Immunology, Southwest Foundation for Biomedical Research San Antonio, Texas; Laboratorio de Imunologia Celular e Molecular, Centro de Pesquisas Rene Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil; Departamento de Parasitologia Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

 

ABSTRACT

Leishmaniavirus (LRV) is a double-stranded RNA virus that infects the protozoa Leishmania and has been identified in numerous strains of Leishmania braziliensis and L. braziliensis guyanensis. In general, the species of Leishmania dictates disease manifestation except in the case of L. braziliensis, which is capable of causing either cutaneous or mucocutaneous leishmaniasis. We wanted to determine 1) the quantity of LRV RNA present in a clinical sample and 2) if infection with LRV was associated with a specific disease manifestation. A real-time reverse transcriptase–polymerase chain reaction assay was used to assay clinical samples for the presence of LRV. Of 47 samples tested, 12 positive samples were obtained from patients with cutaneous lesions, lesions in the process of scarring, and cutaneous scars. This is the first study to examine the prevalence of LRV RNA within a small cohort from Brazil.

Received October 21, 2002. Accepted for publication June 16, 2003.

Acknowledgment: We thank Dr. Raymond Cologna for his reviews and suggestions.

Financial support: This work was supported by National Institutes of Health grant AI28473. Monica M. Ogg received financial support through a virology training grant (T32 AI07522).

Authors’ addresses: Monica M. Ogg and Ricardo Carrion, Jr., Department of Microbiology, University of Texas Health Science Center San Antonio, San Antonio, TX 78229, Telephone: 210-258-9865, Fax: 210-670-3329, E-mails: ogg{at}uthscsa.edu and carrion{at}sfbr.org. Ana Cristina de Carvalho Botelho and Rodrigo Correa-Oliveira, Laboratorio de Imunologia Celular e Molecular, Centro de Pesquisas Rene Rachou, FIOCRUZ, Av. Augusto de Lima 1715, Belo Horizonte, Minas Gerais, Brazil, 30190-002, Telephone: 55-31-3295-3566, Fax: 55-31-3295-3115, E-mail: correa{at}cpqrr.fiocruz.br. Wilson Mayrink, Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil. Jean L. Patterson, Department of Virology and Immunology, Southwest Foundation for Biomedical Research, PO Box 760549, San Antonio, TX 78245-0549, Telephone: 210-258-9431, Fax: 210-670-3329, E-mail: jpatters{at}sfbr.org.

Reprint requests: Jean L. Patterson, Department of Virology and Immunology, Southwest Foundation for Biomedical Research, PO Box 760549, San Antonio, TX 78245-0549.




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