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Am. J. Trop. Med. Hyg., 69(1), 2003, pp. 98-104
Copyright © 2003 by The American Society of Tropical Medicine and Hygiene

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UTILITY OF COMPARATIVE ANCHOR-TAGGED SEQUENCES AS PHYSICAL ANCHORS FOR COMPARATIVE GENOME ANALYSIS AMONG THE CULICIDAE

ERIC W. CHAMBERS, DIANE D. LOVIN, AND DAVID W. SEVERSON
Center for Tropical Disease Research and Training, Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana

The development of comparative genetic maps in multiple species of mosquitoes could prove extremely useful in the search for those genes that contribute to mosquito vector competence or genes associated with other phenotypes of interest. To effectively compare these gene maps, markers must be developed that are based on chromosomal regions conserved throughout the Culicidae. We designed 35 polymerase chain reaction (PCR) primer pairs based upon orthlogous exons in Aedes aegypti and Drosophila melanogaster or Anopheles gambiae. Twenty-three of the primers yielded a single PCR product in at least one dipteran, in addition to Ae. aegypti, when screened with genomic DNA from seven dipterans, including five mosquito species. Eight of the primers amplified a single PCR product in only Ae. aegypti, while four primer pairs gave no PCR product in any species. The 23 successful comparative anchor-tagged sequence primer pairs give broad genome coverage in Ae. aegypti, and more importantly demonstrate an efficient strategy for developing comparative anchor marker loci for any species of Culicidae.

Received December 27, 2002. Accepted for publication April 3, 2003.

Financial support: This work supported by National Institute of Health grants AI-33127 and AI-34337.

Authors’ address: Eric W. Chambers, Diane D. Lovin, and David W. Severson, Center for Tropical Disease Research and Training, Department of Biologic Sciences, University of Notre Dame, Notre Dame, IN 46556, Telephone 574-631-3826, Fax 574-631-7413, E-mail: Severson.1{at}nd.edu




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[Abstract] [Full Text] [PDF]


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Copyright © 2003 by the American Society of Tropical Medicine and Hygiene.