AJTMH Tropical Medicine and Hygiene News
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 69(1), 2003, pp. 98-104
Copyright © 2003 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by CHAMBERS, E. W.
Right arrow Articles by SEVERSON, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by CHAMBERS, E. W.
Right arrow Articles by SEVERSON, D. W.

UTILITY OF COMPARATIVE ANCHOR-TAGGED SEQUENCES AS PHYSICAL ANCHORS FOR COMPARATIVE GENOME ANALYSIS AMONG THE CULICIDAE

ERIC W. CHAMBERS, DIANE D. LOVIN, AND DAVID W. SEVERSON
Center for Tropical Disease Research and Training, Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana

The development of comparative genetic maps in multiple species of mosquitoes could prove extremely useful in the search for those genes that contribute to mosquito vector competence or genes associated with other phenotypes of interest. To effectively compare these gene maps, markers must be developed that are based on chromosomal regions conserved throughout the Culicidae. We designed 35 polymerase chain reaction (PCR) primer pairs based upon orthlogous exons in Aedes aegypti and Drosophila melanogaster or Anopheles gambiae. Twenty-three of the primers yielded a single PCR product in at least one dipteran, in addition to Ae. aegypti, when screened with genomic DNA from seven dipterans, including five mosquito species. Eight of the primers amplified a single PCR product in only Ae. aegypti, while four primer pairs gave no PCR product in any species. The 23 successful comparative anchor-tagged sequence primer pairs give broad genome coverage in Ae. aegypti, and more importantly demonstrate an efficient strategy for developing comparative anchor marker loci for any species of Culicidae.

Received December 27, 2002. Accepted for publication April 3, 2003.

Financial support: This work supported by National Institute of Health grants AI-33127 and AI-34337.

Authors’ address: Eric W. Chambers, Diane D. Lovin, and David W. Severson, Center for Tropical Disease Research and Training, Department of Biologic Sciences, University of Notre Dame, Notre Dame, IN 46556, Telephone 574-631-3826, Fax 574-631-7413, E-mail: Severson.1{at}nd.edu




This article has been cited by other articles:


Home page
 GeneticsHome page
J. R. Anderson, J. R. Schneider, P. R. Grimstad, and D. W. Severson
Quantitative Genetics of Vector Competence for La Crosse Virus and Body Size in Ochlerotatus hendersoni and Ochlerotatus triseriatus Interspecific Hybrids
Genetics, March 1, 2005; 169(3): 1529 - 1539.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the American Society of Tropical Medicine and Hygiene.