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Am. J. Trop. Med. Hyg., 69(1), 2003, pp. 58-59
Copyright © 2003 by The American Society of Tropical Medicine and Hygiene

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A SURVEY FOR SPOTTED FEVER GROUP RICKETTSIAE AND EHRLICHIAE IN AMBLYOMMA VARIEGATUM FROM ST. KITTS AND NEVIS

PATRICK J. KELLY, PIERRE-EDOUARD FOURNIER, PHILIPPE PAROLA, AND DIDIER RAOULT
School of Veterinary Medicine, Ross University, Basseterre, St. Kitts, West Indies; Unité des Rickettsies, Faculté de Médecine, Université de la Méditerranée, Marseille, France

Eighty-nine Amblyomma variegatum ticks were collected from the islands of St. Kitts and Nevis in the Caribbean and preserved in 70% ethanol or local rum. After being washed in sterile water, their DNA was extracted and analyzed by a polymerase chain reaction (PCR) for DNA of spotted fever group rickettsiae and ehrlichiae. None of the tested ticks was positive in a PCR assay using the primers 16S EHRD and 16S EHRR for the 16S rRNA gene of Ehrlichia spp.. Forty-one percent of the A. variegatum (36 of /89 of which 34 [47%] of 72 were adult males, 2 (13%) of 16 were adult females, and 0 (0%) of 1 were nymphs) were positive in a PCR assay using the primer pair 190-70 and 190-701 for the outer membrane protein A (ompA) gene of spotted fever group rickettsiae. All PCR amplification products obtained had 100% sequence homology with Rickettsia africae, the agent of African tick-bite fever.

Received December 11, 2002. Accepted for publication February 26, 2003.

Acknowledgments: We thank Sheldon McMahon and Dr. Patricia Bartlett for providing us with the ticks, and Annick Abeille for her expert technical assistance.

Authors’ addresses: Patrick J. Kelly, School of Veterinary Medicine, Ross University, Basseterre, St. Kitts, West Indies. Pierre-Edouard Fournier, Philippe Parola, and Didier Raoult, Unité des Rickettsies, Faculté de Médecine, Université de la Méditerranée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 5, France.

Reprint requests: Didier Raoult, Unité des Rickettsies, Faculté de Médecine, Université de la Méditerranée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 5, France, Telephone: 33-4-91-38-55-17, Fax: 33-4-91-38-77-72, E-mail: didier.raoult{at}medecine.univ-mrs.fr




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Clin. Microbiol. Rev., October 1, 2005; 18(4): 719 - 756.
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Copyright © 2003 by the American Society of Tropical Medicine and Hygiene.