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IDENTIFICATION OF THE PEOPLE FROM WHOM ENGORGED AEDES AEGYPTI TOOK BLOOD MEALS IN FLORIDA, PUERTO RICO, USING POLYMERASE CHAIN REACTION-BASED DNA PROFILING

We used polymerase chain reaction-based DNA profiling to construct allelic profiles for residents and visitors of 22 houses in Florida, Puerto Rico, and human DNA from blood meals in Aedes aegypti that were collected in those homes. Complete profiles were obtained for 2 days after blood ingestion. Eighteen percent of the meals came from two different people. There was no evidence of meals from 2 people. Eighty percent of the meal sources were identified, > 70% were taken from residents of the collection house, and > 90% were from residents of the study community. Across the community, feeding was non-random with a bias towards young adults and males. Three people accounted for 56% of the meals. Our results confirm that multiple feeding on different people is an important component in the role of Ae. aegypti in dengue virus transmission and help explain the spatial distribution of dengue cases in a previous epidemic in Florida, Puerto Rico.

Received May 13, 2002. Accepted for publication December 4, 2002.

Acknowledgments: We thank the residents of Yanes III, Florida, PR for allowing us to work with them in their homes, and the Puerto Rico Department of Health, the Mayor and Vice-Mayor of Florida (Maggie Guzman and Ivette Romero, respectively), and Rosa Agustini and Dr. Magali Rivera (Centro de Cuidados Primarios, Florida) for their permission to work in Florida and their help in carrying out the study. We also thank Laura Harrington, Amy Morrison, Michael Desena, Geoff Attardo, and Amara Naksathit for help in collecting and processing field specimens, and Michael Catlett, John Hileman, Mariam Khaliqi, and Nazzy Pakpour for help in carrying out laboratory analyses.

Financial support: This study was supported by grant AI-22119 from the National Institutes of Health.

Authors’ addresses: John De Benedictis, John D. Edman, and Thomas W. Scott, Department of Entomology, University of California, Davis, CA 95616. Esther Chow-Shaffer, Department of Entomology, University of Maryland, College Park, MD 20742. Adriana Costero, Medical Entomology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 4 Center Drive, MSC 0425, Bethesda, MD 20892-0425. Gary G. Clark, Dengue Branch, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, PR 00920-3860.

Reprint requests: Thomas W. Scott, Department of Entomology, University of California, 1 Shields Avenue, Davis, CA 95616, Telephone: 530-754-4196, Fax: 530-752-1537, E-mail: twscott{at}ucdavis.edu

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