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GROWTH INHIBITORY EFFECT OF TRICLOSAN ON EQUINE AND BOVINE BABESIA PARASITES

We evaluated the growth inhibitory effect of triclosan, which has recently been reported to inhibit the growth of Plasmodium species and Toxoplasma gondii, on bovine and equine Babesia parasites in in vitro cultures. The growth of Babesia bovis and B. bigemina was significantly inhibited in the presence of 100 µg/ml of triclosan, while B. caballi and B. equi were susceptible to as low as 50 µg/ml. Babesia bigemina and B. caballi were completely cleared as early as on the first and second day of the treatment, respectively. These parasites did not exhibit any growth in the subsequent five-day period of subculture without triclosan. Drug-treated parasites appeared pycnotic and atypically shaped, and ultrastructurally showed pronounced vacuolations, leading to complete destruction of parasites. Light microscopy showed that used concentrations of triclosan showed no toxicity against the host cells. The results suggest that triclosan can be used for chemotherapy of babesiosis.

Received July 29, 2002. Accepted for publication November 25, 2002.

Acknowledgments: We thank Akiko Nagai, Dr. Susanne Walter, and Dr. Badgar Battsetseg for their technical support and advice. This article was presented in part at the 42nd Annual Meeting of Japanese Society of Tropical Medicine, Tokyo, September 2001 (abstract A 16), and the 133rd Meeting of Japanese Society of Veterinary Science, Tokyo, March 2002 (article C-32 VII).

Financial support: This study was supported by Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Sciences.

Authors’ addresses: Sabine Bork, Naoaki Yokoyama, Tomohide Matsuo, Kozo Fujisaki, and Ikuo Igarashi, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. Florencia G. Claveria, Biology Department, College of Science, De La Salle University-Manila, Taft Avenue, Manila, The Philippines.

Reprints requests: Ikuo Igarashi, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan, Telephone: 81-155-49-5641, Fax: 81-155-49-5643, E-mail: igarcpmi{at}obihiro.ac.jp

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