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Am. J. Trop. Med. Hyg., 68(3), 2003, pp. 312-320
Copyright © 2003 by The American Society of Tropical Medicine and Hygiene

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COMPARATIVE EVALUATION OF ELISAS BASED ON TEN RECOMBINANT OR PURIFIED LEISHMANIA ANTIGENS FOR THE SERODIAGNOSIS OF MEDITERRANEAN VISCERAL LEISHMANIASIS

IBTISSEM ABDMOULEH MAALEJ, MEHDI CHENIK, HECHMI LOUZIR, AFIF BEN SALAH, CHOKRI BAHLOUL, FETHI AMRI, AND KOUSSAY DELLAGI
Laboratoire d’immunologie (LAF301) and Laboratoire d’Epidémiologie et d’Ecologie Parasitaire, Institut Pasteur de Tunis, Tunis, Tunisia; WHO Collaborating Center for Research and Training in Immunology and Leishmaniasis; Hôpital Universitaire de Kairouan-Tunisie

This study compared a panel of 10 enzyme-linked immunosorbent assays (ELISAs) for the serodiagnosis of Mediterranean visceral leishmaniasis (MVL). The ELISAs were based on either one of the following Leishmania antigens: crude soluble Leishmania antigens (SLAs), recombinant (r) antigens (namely: rgp63, rK39, gene B protein, rH2A and rH2B histones proteins, rLACK, rPSA-2, r P20) and purified lipophosphoglycan. Most of the test antigens showed good performance (sensitivity > 85%, specificity > 80%). rK39 and SLA-based ELISA gave the best results in terms of sensitivity (100%) and predictive value of the negative (100%). The best specificity (97%) and the best predictive value of the positive (92%) were obtained with rK39. These results show that several Leishmania antigens are suitable to design a diagnostic ELISA of MVL. However, recombinant proteins add little to the classic crude SLA, which still represents a very good and less costly alternative.

Received February 26, 2002. Accepted for publication August 12, 2002.

Acknowledgments: We thank Dr. E. Handman, R. McMaster, S.G. Reed and D.F. Smith for their gift of the purified antigens used in this study. We are grateful to Dr. D.F. Smith and E. Handman for reviewing and commenting on the manuscript.

Financial support: This work was partly supported by the Tunisian Ministry of Scientific Research and Technology, by the MERC Program USAID/NIAID (grant N° N01-A045183), and by the DGXII European Commission INCO-MED Programme (grant N° I C18/CT97/0252).

Reprint requests: Koussay Dellagi, Institut Pasteur de Tunis, 1002 Tunis-Belvédère, Tunisia, Telephone: 216-71-789-608, Fax: 216-71-791-833, E-mail: Koussay.Dellagi{at}pasteur.rns.tn




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