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Am. J. Trop. Med. Hyg., 68(3), 2003, pp. 276-280
Copyright © 2003 by The American Society of Tropical Medicine and Hygiene

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SIMILAR DIAGNOSTIC PERFORMANCE FOR NEUROCYSTICERCOSIS OF THREE GLYCOPROTEIN PREPARATIONS FROM TAENIA SOLIUM METACESTODES

GUIDO E. VILLOTA, DIANA I. GOMEZ, MICHEL VOLCY, ANDRÉS F. FRANCO, EDGAR A. CARDONA, RODRIGO ISAZA, FERNANDO SANZÓN, JUDY M. TEALE, AND BLANCA I. RESTREPO
Molecular Parasitology Group, Corporación para Investigaciones Biológicas, Medellín, Colombia; Facultad de Ciencias Pecuarias, Centro de Estudios en Salud, Universidad de Nariño, San Juan de Pasto, Colombia; Universidad de Antioquia and Hospital Universitario San Vicente de Paul, Medellín, Colombia; Neurology Service, Hospital Pablo Tobón Uribe, Medellín, Colombia; Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas

The detection of antibodies to Taenia solium metacestodes is very important in the differential diagnosis of neurocysticercosis (NCC). In this study, an electroimmunotransfer blot (EITB) assay that uses an elaborate protocol with metacestode glycoproteins as antigens was compared with two other Western blots that use glycoproteins obtained using simpler methods, including an eluate from a lectin column, or the vesicular fluid (VF) of the parasite. The concordance between the three assays was 91% in patients with active NCC and 100% in patients with suspected NCC and previous documentation of negative serology. The specificities for the Western blots and the EITB assay were 98% and 100%, respectively (98% concordance). These data suggest that the simplest of these immunoassays, the one that uses the VF of T. solium metacestodes in a Western blot format, can be reliably used for the serologic diagnosis of NCC in developing countries where access to the EITB assay is difficult.

Received September 2, 2002. Accepted for publication November 18, 2002.

Acknowledgment: We thank the late Dora Luz Gil for technical support.

Financial support: This work was supported by grant TW00953 from the National Institutes of Health, Fogarty International, and the Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología Francisco José de Caldas, COLCIENCIAS.

Authors’ addresses: Guido E. Villota, Laboratorio de Microbiología, Ciudad Universitaria Torobajo, Universidad de Nariño, San Juan de Pasto, Colombia, Telephone: 578-2-722-0861, E-mail: guiervical{at}latinmail.com. Diana I. Gomez, Corporación para Investigaciones Biológicas, Cra. 72A No. 78B-141, Medellín, Colombia, Telephone: 57-4-441-0855, E-mail: artemisa_dg{at}yahoo.com. Michel Volcy and Andrés F. Franco, Hospital Universitario San Vicente de Paul and Departamento de Neurología, Facultad de Medicina, Universidad de Antioquia, CRA 52 No. 62-28, Medellín, Colombia, Telephone: 57-4-263-0253, Fax: 57-4-263-5333, E-mails: medvol98{at}yahoo.com and anferfran{at}geo.net.co. Edgar A. Cardona and Rodrigo Isaza, Hospital Pablo Tobon Uribe Calle 78B No 69-240, Medellín, Colombia, Telephone: 57-4-441-5252, E-mails: eacr{at}epm.net.co and risaza{at}epm.net.co. Fernando Sanzón, Centro de Estudios en Salud, CES, Ciudad Universitaria Torobajo, Universidad de Nariño, San Juan de Pasto, Colombia, Telephone: 57-2-731-2283, Fax: 57-2-731-2283, E-mail: afsanson{at}multipasto.net.co. Judy M. Teale, Department of Microbiology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, Telephone: 210-567-3959, E-mail: teale{at}uthscsa.edu. Blanca I. Restrepo, 417 E. Cardinal Avenue, McAllen, TX 78504, Telephone: 956-664-2124, Fax: 956-664-2248, E-mail: blancos{at}rgv.rr.com or University of Texas Health Science Center Houston-School of Public Health, The University of Texas at Brownsville, 80 Fort Brown SPH, Second Floor Room 2.202, Brownsville, TX 78520, Telephone: 956-554-5165, Fax: 956-554-6674.






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