HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by HARUMAL, P. Articles by KEMP, D. J. Search for Related Content PubMed PubMed Citation Articles by HARUMAL, P. Articles by KEMP, D. J. Related Collections Scabies Medical Entomology

IDENTIFICATION OF A HOMOLOGUE OF A HOUSE DUST MITE ALLERGEN IN A cDNA LIBRARY FROM SARCOPTES SCABIEI VAR. HOMINIS AND EVALUATION OF ITS VACCINE POTENTIAL IN A RABBIT/S. SCABIEI VAR. CANIS MODEL

Sarcoptes scabiei ("itch mite") causes scabies, a disease of considerable human and veterinary significance. Little work has been done at the molecular level because of the difficulty of obtaining mites. We have used mites in skin from the bedding of crusted scabies patients for the construction of a library of 10⁵ cDNAs from S. scabiei var. hominis cloned in the vector pGEX4T-2. We describe the isolation by immunoscreening of 2 clones, one of which (Ssag1) is homologous to and cross-reactive with the house dust mite Euroglyphus maynei allergen M-177, an apolipoprotein from hemolymph. Immunohistochemistry revealed that it is located around the internal organs and cuticle of the mite and in eggs. Although it was not found to be protective in a challenge trial, the rabbits did not exhibit typical crust characteristics. This work shows that it is now possible to conduct such challenge trials with cloned scabies antigens.

Received January 7, 2002. Accepted for publication June 27, 2002.

Acknowledgments: The authors thank Monika Stanczew for assistance with the immunohistochemistry.

Financial support: Work in Australia was supported by the Australian National Health and Medical Research Council (grant 980431) and the Cooperative Research Centre for Aboriginal and Tropical Health. Work in the United States was supported by NIH grant AI17252 from the National Institute of Allergy and Infectious Disease.

Reprint requests: Bart J. Currie, Menzies School of Health Research, P.O. Box 41096, Casuarina, NT 0811, Australia, Telephone: 61-8-89228196, Fax: 61-8-89275187, E-mail: bart{at}menzies.edu.au

Authors’ addresses: Pearly Harumal, Shelley F. Walton, and Bart J. Currie, Menzies School of Health Research, P.O. Box 41096, Casuarina, NT 0811, Australia, Telephone: 61-8-89228196, Fax: 61-8-89275187. Deborah C. Holt and David J. Kemp, The Queensland Institute of Medical Research, The Australian Centre for International and Tropical Health and Nutrition, The University of Queensland, P.O. Royal Brisbane Hospital, Qld 4029, Australia, Telephone: 61-7-33620402, Fax: 61-7-33620104. Marjorie Morgan and Larry G. Arlian, Department of Biologic Sciences, Wright State University, Dayton, OH 45435, Telephone: 937-775-2568, Fax: 937-775-3320. Jurgen Rode, Royal Darwin Hospital, Tiwi, NT 0811, Australia, Telephone: 61-8-89228670, Fax: 61-8-89228843.

This article has been cited by other articles:

				S. F. Walton and B. J. Currie Problems in Diagnosing Scabies, a Global Disease in Human and Animal Populations Clin. Microbiol. Rev., April 1, 2007; 20(2): 268 - 279. [Abstract] [Full Text] [PDF]

				A. DOUGALL, D. C. HOLT, K. FISCHER, B. J. CURRIE, D. J. KEMP, and S. F. WALTON IDENTIFICATION AND CHARACTERIZATION OF SARCOPTES SCABIEI AND DERMATOPHAGOIDES PTERONYSSINUS GLUTATHIONE S-TRANSFERASES: IMPLICATION AS A POTENTIAL MAJOR ALLERGEN IN CRUSTED SCABIES Am J Trop Med Hyg, November 1, 2005; 73(5): 977 - 984. [Abstract] [Full Text] [PDF]