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CAUSED BY INFECTION OF CULTURED HUMAN MONOCYTES WITH DENGUE VIRUS
Dengue (DEN) virus is responsible for one of the most significant viral diseases in tropical countries. Monocytes/macrophages (Mo/M
) are the major target cells for DEN virus. To determine the effects of the interaction between DEN virus and Mo/M, human monocyte cultures were infected with DEN virus type 2. Apoptosis and production of tumor necrosis factor-
(TNF-) and nitric oxide were measured in control and infected cultures. Virus was taken up by phagocytosis, but no membrane-coated pits at the virus attachment sites were observed. Increased number of apoptotic cells and increased production of TNF- were observed in infected monocyte cultures. No increase in production of nitric oxide was observed. These results may be related to early primary viral infection, in which virus could induce apoptosis in monocytes, but monocytes may contribute to host defense mechanisms against virus by viral phagocytosis, phagocytosis of infected apoptotic cells, and the release of proinflammatory cytokines.
Received April 25, 2001. Accepted for publication March 21, 2002.
Acknowledgment: We thank Dr. Duane Gubler (Centers for Disease Control and Prevention, Fort Collins, CO) for the monoclonal antibody to dengue virus type 2 used in the viral immunofluorescence studies.
Reprint requests: Jesus A. Mosquera, Apartado Postal 1151, Maracaibo 4001-A, Zulia, Venezuela, Telephone/Fax: 58-61-597-247, E-mail: mosquera99{at}hotmail.com
Authors’ addresses: Luz M. Espina, Nereida J. Valero, and Janeth M. Hernández: Seccion de Virologia. Instituto de Investigaciones Clinicas Dr. Americo Negrete, Facultad de Medicina, Universidad del Zulia, Apartado Postal 1151, Maracaibo 4001-A, Zulia, Venezuela, Jesús A. Mosquera, Seccion de Inmunologia y Biología Celular, Instituto de Investigaciones Clinicas Dr. Americo Negrete, Facultad de Medicina, Universidad del Zulia, Apartado Postal 1151, Maracaibo 4001-A, Zulia, Venezuela.
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