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Am. J. Trop. Med. Hyg., 68(1), 2003, pp. 44-45
Copyright © 2003 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT: ANCYLOSTOMA CEYLANICUM: EXSHEATHMENT IS NOT REQUIRED FOR SUCCESSFUL CRYOPRESERVATION OF THIRD STAGE HOOKWORM LARVAE

JOHN DUARTE, LISA M. HARRISON, AND MICHAEL CAPPELLO
Child Health Research Center, Departments of Pediatrics, and Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut

 

ABSTRACT

Third-stage larvae (L3) of the human hookworm parasite Ancylostoma ceylanicum were cultured from the feces of infected hamsters and frozen for up to 100 days in liquid nitrogen. Upon thawing, viable larvae were recovered and used to inoculate naive hamsters. The larvae recovered from this second group of hamsters were used to inoculate a third group of naive animals, which demonstrated that the originally frozen larvae were successfully maintained for two full generations following thawing. These data suggest that exsheathment, which has previously been reported to be essential for successful cryopreservation, is not necessary for recovery of viable, infectious A. ceylanicum L3.

Received June 4, 2002. Accepted for publication September 25, 2002.

Acknowledgments: We thank Peter Hotez and John Hawdon (George Washington University, Washington, DC) for providing the A. ceylanicum L3, and Richard Bungiro for his thoughtful comments and suggestions during the course of this work.

Financial support: This work was supported by National Institutes of Health grant AI-47929 (Michael Cappello) and a New Investigator Award from the Burroughs Wellcome Fund (Michael Cappello).

Reprint requests: Michael Cappello, Department of Pediatrics, Yale University School of Medicine, 464 Congress Avenue, New Haven, CT 06520, Telephone: 203-737-4320, Fax: 203-737-5972, E-mail: michael.cappello{at}yale.edu

Authors’ addresses: John Duarte, Lisa M. Harrison, and Michael Cappello, Department of Pediatrics, Yale University School of Medicine, 464 Congress Avenue, New Haven, CT 06520.






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Copyright © 2003 by the American Society of Tropical Medicine and Hygiene.