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Am. J. Trop. Med. Hyg., 68(1), 2003, pp. 40-43
Copyright © 2003 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT: ECHINOCOCCUS GRANULOSUS FROM XINJIANG, PR CHINA: cDNAS ENCODING THE EG95 VACCINE ANTIGEN ARE EXPRESSED IN DIFFERENT LIFE CYCLE STAGES AND ARE CONSERVED IN THE ONCOSPHERE

WENBAO ZHANG, JUN LI, HONG YOU, ZHUANGZHI ZHANG, GULINUL TURSON, ALEX LOUKAS, AND DONALD P. MCMANUS
Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research and University of Queensland, Brisbane, Queensland, Australia; Veterinary Research Institute, Xinjiang Academy of Animal Science, Urumqi, Xinjiang, China.

 

ABSTRACT

The EG95-based vaccine protects sheep from infection with the dog tapeworm Echinococcus granulosus. The EG95 encoding gene is a member of a multigene family, several members of which are expressed in the oncosphere, believed to be the target of immunity induced by the vaccine. E. granulosus exhibits extensive intraspecific (strain) variation, and variability of the eg95 gene in different isolates of E. granulosus may directly impact the effectiveness of the EG95-based vaccine. We analyzed the eg95 gene from E. granulosus collected in Xinjiang, in northwest China, where hydatid disease is hyperendemic. The gene is expressed in oncospheres, protoscoleces, and immature and mature adult worms, and the eg95 gene family was shown to comprise two basic sequence types. Very limited sequence variation was evident in the EG95 protein from oncospheres. This high degree of sequence conservation predicts that the vaccine will continue to be effective in China and elsewhere.

Received April 29, 2002. Accepted for publication September 11, 2002.

Financial support: This work was supported by the Wellcome Trust (WZ and DPM), the Australian National Health and Medical Research Council (DPM), and by Australian International Postgraduate Research (IPRS) and University of Queensland International Postgraduate Research Scholarships (UQIPRS) to WZ.

New nucleotide sequence data reported in this paper have been submitted to the Gen-BankTM database with the accession numbers: AF503596–AF503604.

Reprint requests: Professor Don McManus, Molecular Parasitology Laboratory, Queensland Institute of Medical Research, 300 Herston Road, Brisbane, Queensland 4006, Australia, Telephone: 61-7-3362 0401, Fax: 61-7-3362 0104, E-mail: donM{at}qimr.edu.au

Authors’ addresses: Jun Li, Hong You, Alex Loukas, and Donald P. McManus. Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, The Queensland Institute of Medical Research and The University of Queensland, Brisbane, Queensland 4029. Wenbao Zhang, Zhuangzhi Zhang, and Gulinul Turson, Veterinary Research Institute, Xinjiang Academy of Animal Science, Urumqi, Xinjiang 830000, China.






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