AJTMH ASTMH MEMBERSHIP INFORMATION: astmh@astmh.org
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 67(4), 2002, pp. 388-391
Copyright © 2002 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Basco, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Basco, L.
Related Collections
Right arrow Malaria
American Journal of Tropical Medicine and Hygiene, Vol 67, Issue 4, 388-391
Copyright © 2002 by American Society of Tropical Medicine and Hygiene

Research Articles


Molecular epidemiology of malaria in Cameroon. XIII. Analysis of pfcrt mutations and in vitro chloroquine resistance

LK Basco

The key Lys76Thr amino-acid substitution in Plasmodium falciparum chloroquine-resistance transporter (PfCRT) has been shown to be a reliable marker associated with chloroquine-resistant phenotype in reference clones, but few discordant results have been observed in field isolates. To further examine the relationship between in vitro chloroquine response and pfcrt alleles, the entire exon 2 of the pfcrt gene of 157 Cameroonian isolates was sequenced. All isolates were characterized as having either Cys-72, Met-74, Asn-75, and Lys-76 (wild-type alleles), Cys-72, Ile-74, Glu-75, and Thr-76 (mutant alleles), or mixed alleles. The hypothetical threshold 50% inhibitory concentration (IC50) set at 100 nM distinguished between isolates carrying the wild-type alleles and those with mutant alleles in a large majority of cases (135 of 139 isolates with unmixed pfcrt alleles). Isolates presenting discordant results generally had IC50s within an intermediate range. In vitro chloroquine response of isolates with mixed pfcrt alleles was highly variable. Although discordant results between chloroquine-resistant phenotype and pfcrt alleles were not explained by the immediate adjacent codons, the key Lys76Thr codon may prove to be a highly reliable genetic marker for the epidemiologic monitoring of chloroquine resistance by means of molecular techniques.


This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
L. K. BASCO and P. RINGWALD
MOLECULAR EPIDEMIOLOGY OF MALARIA IN CAMEROON. XXIV. TRENDS OF IN VITRO ANTIMALARIAL DRUG RESPONSES IN YAOUNDE, CAMEROON
Am J Trop Med Hyg, January 1, 2007; 76(1): 20 - 26.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
D. MENARD, D. DJALLE, F. YAPOU, A. MANIRAKIZA, and A. TALARMIN
FREQUENCY DISTRIBUTION OF ANTIMALARIAL DRUG-RESISTANT ALLELES AMONG ISOLATES OF PLASMODIUM FALCIPARUM IN BANGUI, CENTRAL AFRICAN REPUBLIC
Am J Trop Med Hyg, February 1, 2006; 74(2): 205 - 210.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
L. K. BASCO
MOLECULAR EPIDEMIOLOGY OF MALARIA IN CAMEROON. XX. EXPERIMENTAL STUDIES ON VARIOUS FACTORS OF IN VITRO DRUG SENSITIVITY ASSAYS USING FRESH ISOLATES OF PLASMODIUM FALCIPARUM
Am J Trop Med Hyg, May 1, 2004; 70(5): 474 - 480.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. L. Waller, R. A. Muhle, L. M. Ursos, P. Horrocks, D. Verdier-Pinard, A. B. S. Sidhu, H. Fujioka, P. D. Roepe, and D. A. Fidock
Chloroquine Resistance Modulated in Vitro by Expression Levels of the Plasmodium falciparum Chloroquine Resistance Transporter
J. Biol. Chem., August 29, 2003; 278(35): 33593 - 33601.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Society of Tropical Medicine and Hygiene.