AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 66(6), 2002, pp. 804-811
Copyright © 2002 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koekemoer, L.
Right arrow Articles by Coetzee, M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koekemoer, L.
Right arrow Articles by Coetzee, M
Related Collections
Right arrow Mosquitoes
American Journal of Tropical Medicine and Hygiene, Vol 66, Issue 6, 804-811
Copyright © 2002 by American Society of Tropical Medicine and Hygiene

Research Articles


A cocktail polymerase chain reaction assay to identify members of the Anopheles funestus (Diptera: Culicidae) group

LL Koekemoer, L Kamau, RH Hunt, and M Coetzee

Anopheles funestus Giles is a major malaria vector in Africa belonging to a group of species with morphologically similar characteristics. Morphological identification of members of the A. funestus group is difficult because of overlap of distinguishing characteristics in adult or immature stages as well as the necessity to rear isofemale lines to examine larval and egg characters. A rapid rDNA polymerase chain reaction (PCR) method has been developed to accurately identify five members of the A. funestus group. This PCR is based on species-specific primers in the ITS2 region on the rDNA to identify A. funestus (approximately 505bp), Anopheles vaneedeni Gillies and Coetzee (approximately 587bp), Anopheles rivulorum Leeson (approximately 411bp), Anopheles leesoni Evans (approximately 146bp), and Anopheles parensis Gillies (approximately 252bp).


This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
M. A. Zapata, A. V. Cienfuegos, O. I. Quiros, M. L. Quinones, S. Luckhart, and M. M. Correa
Discrimination of Seven Anopheles Species from San Pedro de Uraba, Antioquia, Colombia, by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Its Sequences
Am J Trop Med Hyg, July 1, 2007; 77(1): 67 - 72.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Annan, P. Durand, F. J. Ayala, C. Arnathau, P. Awono-Ambene, F. Simard, F. G. Razakandrainibe, J. C. Koella, D. Fontenille, and F. Renaud
Population genetic structure of Plasmodium falciparum in the two main African vectors, Anopheles gambiae and Anopheles funestus
PNAS, May 8, 2007; 104(19): 7987 - 7992.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
E. A. TEMU, I. KIMANI, N. TUNO, H. KAWADA, J. N. MINJAS, and M. TAKAGI
MONITORING CHLOROQUINE RESISTANCE USING PLASMODIUM FALCIPARUM PARASITES ISOLATED FROM WILD MOSQUITOES IN TANZANIA
Am J Trop Med Hyg, December 1, 2006; 75(6): 1182 - 1187.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
T. W. SCOTT, A. K. GITHEKO, A. FLEISHER, L. C. HARRINGTON, and G. YAN
DNA PROFILING OF HUMAN BLOOD IN ANOPHELINES FROM LOWLAND AND HIGHLAND SITES IN WESTERN KENYA.
Am J Trop Med Hyg, August 1, 2006; 75(2): 231 - 237.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. P. Michel, O. Grushko, W. M. Guelbeogo, N. F. Lobo, N. Sagnon, C. Costantini, and N. J. Besansky
Divergence With Gene Flow in Anopheles funestus From the Sudan Savanna of Burkina Faso, West Africa
Genetics, July 1, 2006; 173(3): 1389 - 1395.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
N. MINAKAWA, S. MUNGA, F. ATIELI, E. MUSHINZIMANA, G. ZHOU, A. K. GITHEKO, and G. YAN
SPATIAL DISTRIBUTION OF ANOPHELINE LARVAL HABITATS IN WESTERN KENYAN HIGHLANDS: EFFECTS OF LAND COVER TYPES AND TOPOGRAPHY
Am J Trop Med Hyg, July 1, 2005; 73(1): 157 - 165.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. Cohuet, I. Dia, F. Simard, M. Raymond, F. Rousset, C. Antonio-Nkondjio, P. H. Awono-Ambene, C. S. Wondji, and D. Fontenille
Gene Flow Between Chromosomal Forms of the Malaria Vector Anopheles funestus in Cameroon, Central Africa, and Its Relevance in Malaria Fighting
Genetics, January 1, 2005; 169(1): 301 - 311.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
C. GARROS, L. L. KOEKEMOER, M. COETZEE, M. COOSEMANS, and S. MANGUIN
A SINGLE MULTIPLEX ASSAY TO IDENTIFY MAJOR MALARIA VECTORS WITHIN THE AFRICAN ANOPHELES FUNESTUS AND THE ORIENTAL AN. MINIMUS GROUPS
Am J Trop Med Hyg, June 1, 2004; 70(6): 583 - 590.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
C. GARROS, L. L. KOEKEMOER, L. KAMAU, T. S. AWOLOLA, W. VAN BORTEL, M. COETZEE, M. COOSEMANS, and S. MANGUIN
RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD FOR THE IDENTIFICATION OF MAJOR AFRICAN AND ASIAN MALARIA VECTORS WITHIN THE ANOPHELES FUNESTUS AND AN. MINIMUS GROUPS
Am J Trop Med Hyg, March 1, 2004; 70(3): 260 - 265.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
A. COHUET, F. SIMARD, J.-C. TOTO, P. KENGNE, M. COETZEE, and D. FONTENILLE
SPECIES IDENTIFICATION WITHIN THE ANOPHELES FUNESTUS GROUP OF MALARIA VECTORS IN CAMEROON AND EVIDENCE FOR A NEW SPECIES
Am J Trop Med Hyg, August 1, 2003; 69(2): 200 - 205.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Society of Tropical Medicine and Hygiene.