AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 66(6), 2002, pp. 700-705
Copyright © 2002 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sutherland, C.
Right arrow Articles by Targett, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sutherland, C.
Right arrow Articles by Targett, G.
Related Collections
Right arrow Malaria
American Journal of Tropical Medicine and Hygiene, Vol 66, Issue 6, 700-705
Copyright © 2002 by American Society of Tropical Medicine and Hygiene

Research Articles


Genetic complexity of Plasmodium falciparum gametocytes isolated from the peripheral blood of treated Gambian children

CJ Sutherland, A Alloueche, L McRobert, R Ord, J Leggat, G Snounou, M Pinder, and GA Targett

The genetic complexity of Plasmodium falciparum gametocytes isolated from Gambian children participating in a controlled trial of anti-malarial therapy was investigated. RNA and DNA were prepared from gametocyte-positive blood, which was also used in transmission experiments with Anopheles gambiae mosquitoes. Amplification by a reverse transcriptase-polymerase chain reaction (RT-PCR) of transcripts from the genes for the ring-infected erythrocyte surface antigen and the 16-kD antigen, which exhibit asexual and sexual stage-specific expression, was used to identify 30 post-treatment gametocyte isolates in which trophozoites persisted below the threshold of detection by microscopy. These included isolates from children who received sulfadoxine/pyrimethamine plus artesunate. Twenty-nine gametocyte-positive isolates that were free of subpatent trophozoites were examined further by PCR amplification of polymorphic genomic loci. We estimate that an average minimum of 2.3 genotypes occurred in these gametocyte-only isolates, and many of these were shown to be infective to mosquitoes. Thus, meiotic recombination between different genotypes is predicted to be a common event in this study area.


This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
C. T. HAPPI, G. O. GBOTOSHO, O. A. FOLARIN, O. M. BOLAJI, A. SOWUNMI, D. E. KYLE, W. MILHOUS, D. F. WIRTH, and A. M. J. ODUOLA
ASSOCIATION BETWEEN MUTATIONS IN PLASMODIUM FALCIPARUM CHLOROQUINE RESISTANCE TRANSPORTER AND P. FALCIPARUM MULTIDRUG RESISTANCE 1 GENES AND IN VIVO AMODIAQUINE RESISTANCE IN P. FALCIPARUM MALARIA-INFECTED CHILDREN IN NIGERIA.
Am J Trop Med Hyg, July 1, 2006; 75(1): 155 - 161.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
E. G. DEMBO, H. T. PHIRI, J. MONTGOMERY, M. E. MOLYNEUX, and S. J. ROGERSON
ARE PLASMODIUM FALCIPARUM PARASITES PRESENT IN PERIPHERAL BLOOD GENETICALLY THE SAME AS THOSE SEQUESTERED IN THE TISSUES?
Am J Trop Med Hyg, May 1, 2006; 74(5): 730 - 732.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. McRobert, P. Preiser, S. Sharp, W. Jarra, M. Kaviratne, M. C. Taylor, L. Renia, and C. J. Sutherland
Distinct Trafficking and Localization of STEVOR Proteins in Three Stages of the Plasmodium falciparum Life Cycle
Infect. Immun., November 1, 2004; 72(11): 6597 - 6602.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
C. J. SUTHERLAND, C. J. DRAKELEY, U. OBISIKE, R. COLEMAN, M. JAWARA, G. A. T. TARGETT, P. MILLIGAN, M. PINDER, and G. WALRAVEN
THE ADDITION OF ARTESUNATE TO CHLOROQUINE FOR TREATMENT OF PLASMODIUM FALCIPARUM MALARIA IN GAMBIAN CHILDREN DELAYS, BUT DOES NOT PREVENT TREATMENT FAILURE
Am J Trop Med Hyg, July 1, 2003; 69(1): 19 - 25.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Society of Tropical Medicine and Hygiene.