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Am. J. Trop. Med. Hyg., 66(5), 2002, pp. 599-604
Copyright © 2002 by The American Society of Tropical Medicine and Hygiene

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American Journal of Tropical Medicine and Hygiene, Vol 66, Issue 5, 599-604
Copyright © 2002 by American Society of Tropical Medicine and Hygiene

Research Articles


Identification of mosquito avian-derived blood meals by polymerase chain reaction-heteroduplex analysis

JH Lee, H Hassan, G Hill, EW Cupp, TB Higazi, CJ Mitchell, Godsey MS Jr, and TR Unnasch

A polymerase chain reaction (PCR) heteroduplex assay (HDA) was developed to identify avian derived mosquito blood meals to the species level. The assay used primers amplifying a fragment of the cytochrome B gene from vertebrate but not invertebrate species. In Culex tarsalis fed on quail, PCR products derived from the quail cytochrome B gene were detected seven days post-engorgement. In an analysis of wild-caught mosquitoes, 85% of blood-fed mosquitoes produced detectable PCR products. Heteroduplex patterns obtained from bird-derived PCR products were found to permit the unambiguous identification of all species examined. No intraspecific variation in HDA patterns was found. The PCR-HDA was used to characterize blood meals in wild caught Cx. tarsalis. Of the 67 blood meals analyzed, 60% were derived from avian sources. Of the avian blood meals, 65% were derived from a single host, the common grackle.


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Copyright © 2002 by the American Society of Tropical Medicine and Hygiene.