AJTMH ASTMH Job Mart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 66(5), 2002, pp. 474-480
Copyright © 2002 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thomas, S.
Right arrow Articles by Wirth, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thomas, S.
Right arrow Articles by Wirth, D.
Related Collections
Right arrow Malaria
American Journal of Tropical Medicine and Hygiene, Vol 66, Issue 5, 474-480
Copyright © 2002 by American Society of Tropical Medicine and Hygiene

Research Articles


In vitro chloroquine susceptibility and PCR analysis of pfcrt and pfmdr1 polymorphisms in Plasmodium falciparum isolates from Senegal

SM Thomas, O Ndir, T Dieng, S Mboup, D Wypij, JH Maguire, and DF Wirth

Chloroquine resistance has been linked to mutations in the pfcrt and pfmdr1 genes of Plasmodium falciparum. To estimate the prevalence of the pfcrt K76T, pfmdr1 N86Y, and pfmdr1 D1246Y polymorphisms, isolates of P. falciparum from Senegal, West Africa, were analyzed, and the results were compared to in vitro chloroquine susceptibility. By the in vitro DELI test, 31% of these samples were resistant to chloroquine. Polymerase chain reaction-based assays and confirmatory sequencing demonstrated the pfcrt T76, pfmdr1 Y86, and pfmdr1 Y1246 alleles in 79%, 31%, and 2% of the isolates, respectively. All three mutant alleles were present in both in vitro susceptible and resistant isolates. On the basis of these findings, it appears that these molecular markers are not consistently predictive of in vitro chloroquine resistance in Senegal.


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
R. K. Mehlotra, G. Mattera, M. J. Bockarie, J. D. Maguire, J. K. Baird, Y. D. Sharma, M. Alifrangis, G. Dorsey, P. J. Rosenthal, D. J. Fryauff, et al.
Discordant Patterns of Genetic Variation at Two Chloroquine Resistance Loci in Worldwide Populations of the Malaria Parasite Plasmodium falciparum
Antimicrob. Agents Chemother., June 1, 2008; 52(6): 2212 - 2222.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
J. URSING, P.-E. KOFOED, A. RODRIGUES, L. ROMBO, and J. P. GIL
PLASMODIUM FALCIPARUM GENOTYPES ASSOCIATED WITH CHLOROQUINE AND AMODIAQUINE RESISTANCE IN GUINEA-BISSAU
Am J Trop Med Hyg, May 1, 2007; 76(5): 844 - 848.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
A. M. Gunasekera, T. Wickramarachchi, D. E. Neafsey, I. Ganguli, L. Perera, P. H. Premaratne, D. Hartl, S. M. Handunnetti, P. V. Udagama-Randeniya, and D. F. Wirth
Genetic Diversity and Selection at the Plasmodium vivax Apical Membrane Antigen-1 (PvAMA-1) Locus in a Sri Lankan Population
Mol. Biol. Evol., April 1, 2007; 24(4): 939 - 947.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
M. MAYXAY, M. BARENDS, A. BROCKMAN, A. JAIDEE, S. NAIR, D. SUDIMACK, T. PONGVONGSA, S. PHOMPIDA, R. PHETSOUVANH, T. ANDERSON, et al.
IN VITRO ANTIMALARIAL DRUG SUSCEPTIBILITY AND PFCRT MUTATION AMONG FRESH PLASMODIUM FALCIPARUM ISOLATES FROM THE LAO PDR (LAOS)
Am J Trop Med Hyg, February 1, 2007; 76(2): 245 - 250.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
H. TINTO, B. SANOU, J.-C. DUJARDIN, J. B. OUEDRAOGO, C. VAN OVERMEIR, A. ERHART, E. VAN MARCK, T. R. GUIGUEMDE, and U. D'ALESSANDRO
USEFULNESS OF THE PLASMODIUM FALCIPARUM CHLOROQUINE RESISTANCE TRANSPORTER T76 GENOTYPE FAILURE INDEX FOR THE ESTIMATION OF IN VIVO CHLOROQUINE RESISTANCE IN BURKINA FASO
Am J Trop Med Hyg, July 1, 2005; 73(1): 171 - 173.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
A. MYRICK, O. SARR, T. DIENG, O. NDIR, S. MBOUP, and D. F. WIRTH
ANALYSIS OF THE GENETIC DIVERSITY OF THE PLASMODIUM FALCIPARUM MULTIDRUG RESISTANCE GENE 5' UPSTREAM REGION
Am J Trop Med Hyg, February 1, 2005; 72(2): 182 - 188.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
C. T. HAPPI, G. O. GBOTOSHO, A. SOWUNMI, C. O. FALADE, D. O. AKINBOYE, L. GERENA, D. E. KYLE, W. MILHOUS, D. F. WIRTH, and A. M. J. ODUOLA
MOLECULAR ANALYSIS OF PLASMODIUM FALCIPARUM RECRUDESCENT MALARIA INFECTIONS IN CHILDREN TREATED WITH CHLOROQUINE IN NIGERIA
Am J Trop Med Hyg, January 1, 2004; 70(1): 20 - 26.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
E. O. OCHONG', I. V. F. VAN DEN BROEK, K. KEUS, and A. NZILA
SHORT REPORT: ASSOCIATION BETWEEN CHLOROQUINE AND AMODIAQUINE RESISTANCE AND ALLELIC VARIATION IN THE PLASMODIUM FALCIPARUM MULTIPLE DRUG RESISTANCE 1 GENE AND THE CHLOROQUINE RESISTANCE TRANSPORTER GENE IN ISOLATES FROM THE UPPER NILE IN SOUTHERN SUDAN
Am J Trop Med Hyg, August 1, 2003; 69(2): 184 - 187.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. Nzila, E. Mberu, P. Bray, G. Kokwaro, P. Winstanley, K. Marsh, and S. Ward
Chemosensitization of Plasmodium falciparum by Probenecid In Vitro
Antimicrob. Agents Chemother., July 1, 2003; 47(7): 2108 - 2112.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Society of Tropical Medicine and Hygiene.