AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 65(6), 2001, pp. 907-911
Copyright © 2001 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hamburger, J
Right arrow Articles by Ruppel, A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hamburger, J
Right arrow Articles by Ruppel, A
Related Collections
Right arrow Schistosomiasis
Right arrow Genetic Epidemiology
American Journal of Tropical Medicine and Hygiene, Vol 65, Issue 6, 907-911
Copyright © 2001 by American Society of Tropical Medicine and Hygiene

Research Articles


Polymerase chain reaction assay based on a highly repeated sequence of Schistosoma haematobium: a potential tool for monitoring schistosome-infested water

J Hamburger, He-Na, I Abbasi, RM Ramzy, J Jourdane, and A Ruppel

We have cloned from Schistosoma haematobium genome a repeated sequence, the DraI repeated sequence, which consists of tandemly arranged 121-bp-long units and which is highly abundant (approximately 15% of the S. haematobium genome). By these features, the DraI repeat is similar to the Sm1-7 sequence of Schistosoma mansoni previously described by us. However, their nucleotide sequences are profoundly different. Polymerase chain reaction (PCR) primers were designed on the basis of the DraI sequence information and were used in a PCR assay by which as little as 10 fg of schistosomal DNA as well as individual cercariae were detected. The DraI repeat cross-hybridized with DNA from Schistosoma bovis, Schistosoma magrebowiei, Schistosoma mattheei, Schistosoma curassoni, and Schistosoma intercalatum, but not with DNA from S. mansoni nor from Trichobilharzia ocellata and Echinostoma sp. A potential value of this PCR assay is suggested for monitoring free-living cercariae and infected snails only in bodies free of cross-hybridizing species.


This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
I. ABBASI, C. H. KING, R. F. STURROCK, C. KARIUKI, E. MUCHIRI, and J. HAMBURGER
DIFFERENTIATION OF SCHISTOSOMA HAEMATOBIUM FROM RELATED SCHISTOSOMES BY PCR AMPLIFYING AN INTER-REPEAT SEQUENCE
Am J Trop Med Hyg, May 1, 2007; 76(5): 950 - 955.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
J. HAMBURGER, O. HOFFMAN, H. C. KARIUKI, E. M. MUCHIRI, J. H. OUMA, D. K. KOECH, R. F. STURROCK, and C. H. KING
LARGE-SCALE, POLYMERASE CHAIN REACTION-BASED SURVEILLANCE OF SCHISTOSOMA HAEMATOBIUM DNA IN SNAILS FROM TRANSMISSION SITES IN COASTAL KENYA: A NEW TOOL FOR STUDYING THE DYNAMICS OF SNAIL INFECTION
Am J Trop Med Hyg, December 1, 2004; 71(6): 765 - 773.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
J. Pardo, C. Carranza, M. C. Turrientes, J. L. Perez Arellano, R. Lopez Velez, V. Ramajo, and A. Muro
Utility of Schistosoma bovis Adult Worm Antigens for Diagnosis of Human Schistosomiasis by Enzyme-Linked Immunosorbent Assay and Electroimmunotransfer Blot Techniques
Clin. Vaccine Immunol., November 1, 2004; 11(6): 1165 - 1170.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
I. ABBASI, A. BRANZBURG, M. CAMPOS-PONCE, S. K. A. HAFEZ, F. RAOUL, P. S. CRAIG, and J. HAMBURGER
COPRO-DIAGNOSIS OF ECHINOCOCCUS GRANULOSUS INFECTION IN DOGS BY AMPLIFICATION OF A NEWLY IDENTIFIED REPEATED DNA SEQUENCE
Am J Trop Med Hyg, September 1, 2003; 69(3): 324 - 330.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the American Society of Tropical Medicine and Hygiene.