AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 65(4), 2001, pp. 362-365
Copyright © 2001 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Itoh, M
Right arrow Articles by Kimura, E
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Itoh, M
Right arrow Articles by Kimura, E
Related Collections
Right arrow Travel Medicine
Right arrow Filariasis
American Journal of Tropical Medicine and Hygiene, Vol 65, Issue 4, 362-365
Copyright © 2001 by American Society of Tropical Medicine and Hygiene

Research Articles


Sensitive and specific enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection in urine samples

M Itoh, MV Weerasooriya, G Qiu, NK Gunawardena, MT Anantaphruti, S Tesana, P Rattanaxay, Y Fujimaki, and E Kimura

We developed an enzyme-linked immunosorbent assay (ELISA) that detects filaria-specific immunoglobulin G4 antibodies in unconcentrated urine. The ELISA was positive in 87 of 91 (95.6%) urine samples collected from people with Wuchereria bancrofti microfilariae, antigen, or both. Of 298 urine samples collected in Thailand, Lao People's Democratic Republic, and Japan, where no human filariasis is known, 295 (99.0%) were negative by ELISA. Various intestinal nematode and fluke infections did not interfere with the ELISA. Urine samples with sodium azide could be kept at 37 degrees C for 4 weeks, and the time of urine collection did not influence ELISA results. This ELISA can be used to identify endemic foci of filariasis.


This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
M. Itoh, W. Wu, D. Sun, L. Yao, Z. Li, M. Z. Islam, R. Chen, K. Zhang, F. Wang, S. Zhu, et al.
Confirmation of Elimination of Lymphatic Filariasis by an IgG4 Enzyme-Linked Immunosorbent Assay with Urine Samples in Yongjia, Zhejiang Province and Gaoan, Jiangxi Province, People's Republic of China
Am J Trop Med Hyg, August 1, 2007; 77(2): 330 - 333.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
H. TAKAGI, M. Z. ISLAM, M. ITOH, A. U. ISLAM, A. R. M. SAIFUDDIN EKRAM, S. M. HUSSAIN, Y. HASHIGUCHI, and E. KIMURA
PRODUCTION OF RECOMBINANT KINESIN-RELATED PROTEIN OF LEISHMANIA DONOVANI AND ITS APPLICATION IN THE SERODIAGNOSIS OF VISCERAL LEISHMANIASIS
Am J Trop Med Hyg, May 1, 2007; 76(5): 902 - 905.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
L. Solano-Gallego, A. Rodriguez, L. Iniesta, M. Arboix, M. Portus, and J. Alberola
Detection of Anti-Leishmania Immunoglobulin G Antibodies in Urine Specimens of Dogs with Leishmaniasis
Clin. Vaccine Immunol., September 1, 2003; 10(5): 849 - 855.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
M. V. WEERASOORIYA, M. ITOH, M. Z. ISLAM, X.-G. QIU, Y. FUJIMAKI, and E. KIMURA
PREVALENCE AND LEVELS OF FILARIA-SPECIFIC URINARY IgG4 AMONG CHILDREN LESS THAN FIVE YEARS OF AGE AND THE ASSOCIATION OFANTIBODY POSITIVITY BETWEEN CHILDREN AND THEIR MOTHERS
Am J Trop Med Hyg, April 1, 2003; 68(4): 465 - 468.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
M. Z. Islam, M. Itoh, S. M. Shamsuzzaman, R. Mirza, F. Matin, I. Ahmed, A. K. M. Shamsuzzaman Choudhury, M. A. Hossain, X.-G. Qiu, N. Begam, et al.
Diagnosis of Visceral Leishmaniasis by Enzyme-Linked Immunosorbent Assay Using Urine Samples
Clin. Vaccine Immunol., July 1, 2002; 9(4): 789 - 794.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the American Society of Tropical Medicine and Hygiene.