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Am. J. Trop. Med. Hyg., 65(1), 2001, pp. 10-12
Copyright © 2001 by The American Society of Tropical Medicine and Hygiene

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American Journal of Tropical Medicine and Hygiene, Vol 65, Issue 1, 10-12
Copyright © 2001 by American Society of Tropical Medicine and Hygiene

Research Articles


Differentiation between non-virulent and virulent Burkholderia pseudomallei with monoclonal antibodies to the Ara+ or Ara- biotypes

C Thepthai, T Dharakul, S Smithikarn, S Trakulsomboon, and S Songsivilai

Burkholderia pseudomallei is the causative agent of melioidosis, a fatal tropical infectious disease endemic in Southeast Asia. Environmental isolates of B. pseudomallei have two distinctive biotypes. Some soil isolates are arabinose-assimilators (Ara+ biotype) and are non-virulent in experimental animals. The others cannot assimilate arabinose (Ara- biotype) and are virulent in experimental animals. The Ara- biotype is found in almost all B. pseudomallei clinical isolates. In the present study, a panel of eight monoclonal antibodies that agglutinate the bacteria were produced and tested. The first group, Bps-D2, -D3, -D5, -L1, and -L2 agglutinated 100% of Ara+ clinical and soil isolates of B. pseudomallei. Another group Bps-A1, -A2, and -D1 agglutinated 92.9% and 90.9% of Ara- clinical and soil isolates, respectively. This panel of monoclonal antibodies may be useful for rapid differentiation between non-virulent Ara+ and virulent Ara- B. pseudomallei.


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T. J. J. Inglis, A. Merritt, G. Chidlow, M. Aravena-Roman, and G. Harnett
Comparison of Diagnostic Laboratory Methods for Identification of Burkholderia pseudomallei
J. Clin. Microbiol., May 1, 2005; 43(5): 2201 - 2206.
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Copyright © 2001 by the American Society of Tropical Medicine and Hygiene.