AJTMH ASTMH MEMBERSHIP INFORMATION: astmh@astmh.org
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 62(4), 2000, pp. 427-433
Copyright © 2000 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de Lara Capurro, M
Right arrow Articles by James, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Lara Capurro, M
Right arrow Articles by James, A.
Related Collections
Right arrow Plasmodium
Right arrow Malaria
American Journal of Tropical Medicine and Hygiene, Vol 62, Issue 4, 427-433
Copyright © 2000 by American Society of Tropical Medicine and Hygiene

Research Articles


Virus-expressed, recombinant single-chain antibody blocks sporozoite infection of salivary glands in Plasmodium gallinaceum-infected Aedes aegypti

M de Lara Capurro, J Coleman, BT Beerntsen, KM Myles, KE Olson, E Rocha, AU Krettli, and AA James

Transgenic mosquitoes resistant to malaria parasites are being developed to test the hypothesis that they may be used to control disease transmission. We have developed an effector portion of an antiparasite gene that can be used to test malaria resistance in transgenic mosquitoes. Mouse monoclonal antibodies that recognize the circumsporozoite protein of Plasmodium gallinaceum can block sporozoite invasion of Aedes aegypti salivary glands. An anti-circumsporozoite monoclonal antibody, N2H6D5, whose corresponding heavy- and light-chain gene variable regions were engineered as a single-chain antibody construct, binds to P. gallinaceum sporozoites and prevents infection of Ae. aegypti salivary glands when expressed from a Sindbis virus. Mean intensities of sporozoite infections of salivary glands in mosquitoes expressing N2scFv were reduced as much as 99.9% when compared to controls.


This article has been cited by other articles:


Home page
 J. Exp. Biol.Home page
B. J. Huho, K. R. Ng'habi, G. F. Killeen, G. Nkwengulila, B. G. J. Knols, and H. M. Ferguson
Nature beats nurture: a case study of the physiological fitness of free-living and laboratory-reared male Anopheles gambiae s.l.
J. Exp. Biol., August 15, 2007; 210(16): 2939 - 2947.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
N. JASINSKIENE, J. COLEMAN, A. ASHIKYAN, M. SALAMPESSY, O. MARINOTTI, and A. A. JAMES
GENETIC CONTROL OF MALARIA PARASITE TRANSMISSION: THRESHOLD LEVELS FOR INFECTION IN AN AVIAN MODEL SYSTEM
Am J Trop Med Hyg, June 1, 2007; 76(6): 1072 - 1078.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
C.-H. Chen, H. Huang, C. M. Ward, J. T. Su, L. V. Schaeffer, M. Guo, and B. A. Hay
A Synthetic Maternal-Effect Selfish Genetic Element Drives Population Replacement in Drosophila
Science, April 27, 2007; 316(5824): 597 - 600.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
M. A. Riehle, P. Srinivasan, C. K. Moreira, and M. Jacobs-Lorena
Towards genetic manipulation of wild mosquito populations to combat malaria: advances and challenges
J. Exp. Biol., November 1, 2003; 206(21): 3809 - 3816.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
A. A. James
Blocking malaria parasite invasion of mosquito salivary glands
J. Exp. Biol., November 1, 2003; 206(21): 3817 - 3821.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. A. Moreira, J. Ito, A. Ghosh, M. Devenport, H. Zieler, E. G. Abraham, A. Crisanti, T. Nolan, F. Catteruccia, and M. Jacobs-Lorena
Bee Venom Phospholipase Inhibits Malaria Parasite Development in Transgenic Mosquitoes
J. Biol. Chem., October 18, 2002; 277(43): 40839 - 40843.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
L. Alphey, C. B. Beard, P. Billingsley, M. Coetzee, A. Crisanti, C. Curtis, P. Eggleston, C. Godfray, J. Hemingway, M. Jacobs-Lorena, et al.
Malaria Control with Genetically Manipulated Insect Vectors
Science, October 4, 2002; 298(5591): 119 - 121.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the American Society of Tropical Medicine and Hygiene.