AJTMH Tropical Medicine and Hygiene News
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 61(6), 1999, pp. 893-897
Copyright © 1999 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Balmaseda, A
Right arrow Articles by Harris, E
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Balmaseda, A
Right arrow Articles by Harris, E
Related Collections
Right arrow Zoonotic Diseases
Right arrow Dengue
Right arrow Genetic Epidemiology
American Journal of Tropical Medicine and Hygiene, Vol 61, Issue 6, 893-897
Copyright © 1999 by American Society of Tropical Medicine and Hygiene

Research Articles


Application of molecular typing techniques in the 1998 dengue epidemic in Nicaragua

A Balmaseda, E Sandoval, L Perez, CM Gutierrez, and E Harris

This report presents the results of applying the reverse transcriptase-polymerase chain reaction (RT-PCR) to the analysis of clinical specimens during the 1998 dengue epidemic in Nicaragua. The RT-PCR was validated through comparison with viral isolation, resulting in a sensitivity of 100% and a specificity of 90%. In-country application of the RT-PCR permitted the rapid identification of dengue-3 virus as the cause of the epidemic at the beginning of 1998 and the detection of the reintroduction of dengue-2 virus in the middle of the year. Nineteen isolates of dengue-3 and one of dengue-2 were characterized using the restriction site-specific (RSS)-PCR technique. This showed that the dengue-3 strain belonged to the "Sri Lanka" subtype and that the dengue-2 strain belonged to the "Jamaica" subtype, both of which have been associated with hemorrhagic dengue in the Americas. The application of these simple PCR-based strain typing methods in a country endemic for dengue virus infections can help to characterize the transmission dynamics of this important emerging infectious disease problem and provide this information to local health authorities in a timely manner so that appropriate control measures can be implemented.


This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
C. Rocha, S. Silva, A. Gordon, S. N. Hammond, D. Elizondo, A. Balmaseda, and E. Harris
Improvement in Hospital Indicators after Changes in Dengue Case Management in Nicaragua
Am J Trop Med Hyg, August 1, 2009; 81(2): 287 - 292.
[Abstract] [Full Text] [PDF]

Home page
 Am J EpidemiolHome page
G. Kuan, A. Gordon, W. Aviles, O. Ortega, S. N. Hammond, D. Elizondo, A. Nunez, J. Coloma, A. Balmaseda, and E. Harris
The Nicaraguan Pediatric Dengue Cohort Study: Study Design, Methods, Use of Information Technology, and Extension to Other Infectious Diseases
Am. J. Epidemiol., July 1, 2009; 170(1): 120 - 129.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
V. H. AQUINO, E. ANATRIELLO, P. F. GONCALVES, E. V. DA SILVA, P. F. C. VASCONCELOS, D. S. VIEIRA, W. C. BATISTA, M. L. BOBADILLA, C. VAZQUEZ, M. MORAN, et al.
MOLECULAR EPIDEMIOLOGY OF DENGUE TYPE 3 VIRUS IN BRAZIL AND PARAGUAY, 2002-2004.
Am J Trop Med Hyg, October 1, 2006; 75(4): 710 - 715.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
A. BALMASEDA, S. N. HAMMOND, L. PEREZ, Y. TELLEZ, S. I. SABORIO, J. C. MERCADO, R. CUADRA, J. ROCHA, M. A. PEREZ, S. SILVA, et al.
SEROTYPE-SPECIFIC DIFFERENCES IN CLINICAL MANIFESTATIONS OF DENGUE.
Am J Trop Med Hyg, March 1, 2006; 74(3): 449 - 456.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
S. N. HAMMOND, A. BALMASEDA, L. PEREZ, Y. TELLEZ, S. I. SABORIO, J. C. MERCADO, E. VIDEA, Y. RODRIGUEZ, M. A. PEREZ, R. CUADRA, et al.
DIFFERENCES IN DENGUE SEVERITY IN INFANTS, CHILDREN, AND ADULTS IN A 3-YEAR HOSPITAL-BASED STUDY IN NICARAGUA
Am J Trop Med Hyg, December 1, 2005; 73(6): 1063 - 1070.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A.-M. Helt and E. Harris
S-Phase-Dependent Enhancement of Dengue Virus 2 Replication in Mosquito Cells, but Not in Human Cells
J. Virol., November 1, 2005; 79(21): 13218 - 13230.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
C. C. CARLOS, K. OISHI, M. T. D. D. CINCO, C. A. MAPUA, S. INOUE, D. J. M. CRUZ, M. A. M. PANCHO, C. Z. TANIG, R. R. MATIAS, K. MORITA, et al.
COMPARISON OF CLINICAL FEATURES AND HEMATOLOGIC ABNORMALITIES BETWEEN DENGUE FEVER AND DENGUE HEMORRHAGIC FEVER AMONG CHILDREN IN THE PHILIPPINES
Am J Trop Med Hyg, August 1, 2005; 73(2): 435 - 440.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
C. N. Peyrefitte, P. Couissinier-Paris, V. Mercier-Perennec, M. Bessaud, J. Martial, N. Kenane, J.-P. A. Durand, and H. J. Tolou
Genetic Characterization of Newly Reintroduced Dengue Virus Type 3 in Martinique (French West Indies)
J. Clin. Microbiol., November 1, 2003; 41(11): 5195 - 5198.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
N. Y. Uzcategui, G. Comach, D. Camacho, M. Salcedo, M. Cabello de Quintana, M. Jimenez, G. Sierra, R. Cuello de Uzcategui, W. S. James, S. Turner, et al.
Molecular epidemiology of dengue virus type 3 in Venezuela
J. Gen. Virol., June 1, 2003; 84(6): 1569 - 1575.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
A. Balmaseda, M. G. Guzman, S. Hammond, G. Robleto, C. Flores, Y. Tellez, E. Videa, S. Saborio, L. Perez, E. Sandoval, et al.
Diagnosis of Dengue Virus Infection by Detection of Specific Immunoglobulin M (IgM) and IgA Antibodies in Serum and Saliva
Clin. Vaccine Immunol., March 1, 2003; 10(2): 317 - 322.
[Abstract] [Full Text] [PDF]

Home page
 BMJHome page
E. Harris and M. Tanner
Health technology transfer
BMJ, September 30, 2000; 321(7264): 817 - 820.
[Full Text]

Home page
 J. Virol.Home page
M. S. Diamond, D. Edgil, T. G. Roberts, B. Lu, and E. Harris
Infection of Human Cells by Dengue Virus Is Modulated by Different Cell Types and Viral Strains
J. Virol., September 1, 2000; 74(17): 7814 - 7823.
[Abstract] [Full Text]

Home page
 J. Virol.Home page
M. S. Diamond, T. G. Roberts, D. Edgil, B. Lu, J. Ernst, and E. Harris
Modulation of Dengue Virus Infection in Human Cells by Alpha, Beta, and Gamma Interferons
J. Virol., June 1, 2000; 74(11): 4957 - 4966.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Society of Tropical Medicine and Hygiene.