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Am. J. Trop. Med. Hyg., 61(4), 1999, pp. 652-653
Copyright © 1999 by The American Society of Tropical Medicine and Hygiene

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American Journal of Tropical Medicine and Hygiene, Vol 61, Issue 4, 652-653
Copyright © 1999 by American Society of Tropical Medicine and Hygiene

Research Articles


Short report: improved diagnosis and follow-up of canine leishmaniasis using amastigote-based indirect immunofluorescence

FJ Fernandez-Perez, S Mendez, C de la Fuente, MT Gomez-Munoz, M Cuquerella, and JM Alunda

A comparison of an indirect immunofluorescence test using promastigotes (IFATp) or cultured amastigotes (IFATa) in the diagnosis and follow-up of canine leishmaniasis caused by Leishmania infantum was carried out. Results obtained with both diagnostic methods were in good agreement although the IFATa titration was more sensitive than the currently used IFATp without losing specificity. The higher sensitivity of the amastigote-based IFAT resulted in an earlier diagnosis in the absence of clinical signs. Both methods showed comparable results for monitoring the clinical evolution of naturally infected and treated (meglumine antimoniate plus allopurinol) dogs.


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S. Bashaye, N. Nombela, D. Argaw, A. Mulugeta, M. Herrero, J. Nieto, C. Chicharro, C. Canavate, P. Aparicio, I. D. Velez, et al.
Risk Factors for Visceral Leishmaniasis in a New Epidemic Site in Amhara Region, Ethiopia
Am J Trop Med Hyg, July 1, 2009; 81(1): 34 - 39.
[Abstract] [Full Text] [PDF]


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