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Am. J. Trop. Med. Hyg., 61(4), 1999, pp. 648-651
Copyright © 1999 by The American Society of Tropical Medicine and Hygiene

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American Journal of Tropical Medicine and Hygiene, Vol 61, Issue 4, 648-651
Copyright © 1999 by American Society of Tropical Medicine and Hygiene

Research Articles


Fasciola gigantica-specific antigens: purification by a continuous-elution method and its evaluation for the diagnosis of human fascioliasis

W Maleewong, C Wongkham, PM Intapan, and V Pipitgool

Immunodominant antigens of an approximate molecular mass of 27 kD were obtained from an excretory-secretory product of adult Fasciola gigantica by a continuous-elution method. An indirect ELISA using the antigens obtained by this relatively simple procedure was developed for detecting specific antibodies from patients infected with F. gigantica. Sera from patients with other parasitic infections, healthy volunteers, and cholangiocarcinoma were also analyzed. The sensitivity, specificity, and positive and negative predictive values for this ELISA using the fractionated antigens were 100%. The data indicated a possible correlation of antibodies to F. gigantica with cholangiocarcinoma.


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Copyright © 1999 by the American Society of Tropical Medicine and Hygiene.