AJTMH ASTMH Job Mart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 61(3), 1999, pp. 482-487
Copyright © 1999 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Raj, V.
Right arrow Articles by Stuart, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Raj, V.
Right arrow Articles by Stuart, K.
Related Collections
Right arrow Leishmaniasis
American Journal of Tropical Medicine and Hygiene, Vol 61, Issue 3, 482-487
Copyright © 1999 by American Society of Tropical Medicine and Hygiene

Research Articles


Serodiagnosis of leishmaniasis with recombinant ORFF antigen

VS Raj, A Ghosh, VS Dole, R Madhubala, PJ Myler, and KD Stuart

The serodiagnostic potential of recombinant ORFF protein (rORFF) from Leishmania infantum was assessed by ELISA. Of 49 sera from confirmed cases of visceral leishmaniasis (VL), all were seropositive using 5 ng of rORFF and serum diluted 1:20, while only 38 were positive with 500 ng of soluble antigen (SA) and 44 were positive by a direct agglutination test. There was also a positive correlation between spleen size and level of seropositivity with rORFF or SA. The reciprocal endpoint titer with rORFF was 1,280 for sera from VL patients, but < 20 with sera from malaria, filariasis, and tuberculosis patients, as well as with sera from healthy individuals from endemic and non-endemic areas. Sera from 10 confirmed cutaneous leishmaniasis cases from Turkey were negative or only weakly positive with rORFF although 9 were positive with SA. Thus, rORFF protein appears useful as a sensitive reagent for the differential diagnosis of VL caused by the Leishmania donovani complex.


This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
M. Z. Islam, M. Itoh, H. Takagi, A. U. Islam, A. R. M. S. Ekram, A. Rahman, A. Takesue, Y. Hashiguchi, and E. Kimura
Enzyme-linked Immunosorbent Assay to Detect Urinary Antibody Against Recombinant rKRP42 Antigen Made from Leishmania donovani for the Diagnosis of Visceral Leishmaniasis
Am J Trop Med Hyg, October 1, 2008; 79(4): 599 - 604.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
H. TAKAGI, M. Z. ISLAM, M. ITOH, A. U. ISLAM, A. R. M. SAIFUDDIN EKRAM, S. M. HUSSAIN, Y. HASHIGUCHI, and E. KIMURA
PRODUCTION OF RECOMBINANT KINESIN-RELATED PROTEIN OF LEISHMANIA DONOVANI AND ITS APPLICATION IN THE SERODIAGNOSIS OF VISCERAL LEISHMANIASIS
Am J Trop Med Hyg, May 1, 2007; 76(5): 902 - 905.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
A. Boarino, A. Scalone, L. Gradoni, E. Ferroglio, F. Vitale, R. Zanatta, M. G. Giuffrida, and S. Rosati
Development of Recombinant Chimeric Antigen Expressing Immunodominant B Epitopes of Leishmania infantum for Serodiagnosis of Visceral Leishmaniasis
Clin. Vaccine Immunol., May 1, 2005; 12(5): 647 - 653.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
M. Z. ISLAM, M. ITOH, R. MIRZA, I. AHMED, A. R. M. S. EKRAM, A. H. SARDER, S. M. SHAMSUZZAMAN, Y. HASHIGUCHI, and E. KIMURA
DIRECT AGGLUTINATION TEST WITH URINE SAMPLES FOR THE DIAGNOSIS OF VISCERAL LEISHMANIASIS
Am J Trop Med Hyg, January 1, 2004; 70(1): 78 - 82.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
R. Barbosa-de-Deus, M. L. dos Mares-Guia, A. Z. Nunes, K. M. Costa, R. G. Junqueira, W. Mayrink, O. Genaro, and C. A. P. Tavares
Leishmania major-Like Antigen for Specific and Sensitive Serodiagnosis of Human and Canine Visceral Leishmaniasis
Clin. Vaccine Immunol., November 1, 2002; 9(6): 1361 - 1366.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
M. Z. Islam, M. Itoh, S. M. Shamsuzzaman, R. Mirza, F. Matin, I. Ahmed, A. K. M. Shamsuzzaman Choudhury, M. A. Hossain, X.-G. Qiu, N. Begam, et al.
Diagnosis of Visceral Leishmaniasis by Enzyme-Linked Immunosorbent Assay Using Urine Samples
Clin. Vaccine Immunol., July 1, 2002; 9(4): 789 - 794.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Society of Tropical Medicine and Hygiene.