AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 61(1), 1999, pp. 103-108
Copyright © 1999 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ranjit, M.
Right arrow Articles by Sharma, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ranjit, M.
Right arrow Articles by Sharma, Y.
Related Collections
Right arrow Plasmodium
Right arrow Epidemiology
Right arrow Malaria
American Journal of Tropical Medicine and Hygiene, Vol 61, Issue 1, 103-108
Copyright © 1999 by American Society of Tropical Medicine and Hygiene

Research Articles


Genetic polymorphism of falciparum malaria vaccine candidate antigen genes among field isolates in India

MR Ranjit and YD Sharma

The present study was designed to investigate the genetic diversity of Plasmodium falciparum among field isolates from India. A total of 71 clinical isolates were analyzed by the polymerase chain reaction (PCR) for the amplification of repeat regions of malaria vaccine candidate antigen genes, i.e., merozoite surface antigen-1 (MSA-1), MSA-2, and circumsporozoite protein (CSP). All three genes showed variation; MSA-2 has the maximum number of 10 variant forms while MSA-1 and CSP had 8 and 6 variants, respectively. Some variant forms were more common than others among the clinical isolates. There were mixed alleles for each gene in several (27 of 71) cases. The MSA-2 gene showed the maximum number of cases with mixed alleles (22 of 65 [33.85%]) compared with MSA-1 (10 of 68 [14.7%]) and CSP (10 of 65 [15.38%]). Fifty-five (88.7%) of 62 clinical isolates of P. falciparum showed a different genotype. The malaria hyperendemic region (Orissa) not only showed the maximum number of variant forms of each gene but also the maximum number of cases with mixed alleles compared with the non-hyperendemic regions (Madhya Pradesh and Rajasthan). The presence of such large numbers of P. falciparum strains in India should be taken into account in future malaria vaccine programs.


This article has been cited by other articles:


Home page
 Clin. Microbiol. Rev.Home page
W. E. Collins and G. M. Jeffery
Plasmodium malariae: Parasite and Disease
Clin. Microbiol. Rev., October 1, 2007; 20(4): 579 - 592.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
W. E. Collins and G. M. Jeffery
Plasmodium ovale: Parasite and Disease
Clin. Microbiol. Rev., July 1, 2005; 18(3): 570 - 581.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Society of Tropical Medicine and Hygiene.