AJTMH HINARI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 60(3), 1999, pp. 493-497
Copyright © 1999 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Attallah, A.
Right arrow Articles by El Ghawalby, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Attallah, A.
Right arrow Articles by El Ghawalby, N.
Related Collections
Right arrow Schistosomiasis
Right arrow Diagnosis
Right arrow Epidemiology
American Journal of Tropical Medicine and Hygiene, Vol 60, Issue 3, 493-497
Copyright © 1999 by American Society of Tropical Medicine and Hygiene

Research Articles


Immunochemical characterization and diagnostic potential of a 63-kilodalton Schistosoma antigen

AM Attallah, E Yones, H Ismail, SA El Masry, A Tabll, AA Elenein, and NA El Ghawalby

Schistosoma circulating antigens were used for the detection of active infection. Anti-S. mansoni IgG2a monoclonal antibody (MAb) designated C5C4 was generated. The target epitope of this MAb was detected in adult worms, eggs, and cercariae antigenic extracts of S. mansoni and S. haematobium, had a molecular size of 63 kD, and was not detected in Fasciola hepatica and Ascaris. In addition, a 50-kD degradation product was identified only in the urine of infected individuals. Analysis by high-performance liquid chromatography of the purified antigen demonstrated only one peak. The 63-kD antigen was characterized as a protein containing 40.4% hydrophobic, 7.5% acidic, and 8.8% basic amino acids. The C5C4 MAb was used in a Fast Dot-ELISA for rapid and simple diagnosis of human schistosomiasis. The 63-kD circulating antigen was detected in 92% of urine samples from 330 S. mansoni-infected individuals, with 16% false-positive results among 130 noninfected individuals.


This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
A. M. ATTALLAH, G. E. GHANEM, H. ISMAIL, and A. M. EL WASEEF
PLACENTAL AND ORAL DELIVERY OF SCHISTOSOMA MANSONI ANTIGEN FROM INFECTED MOTHERS TO THEIR NEWBORNS AND CHILDREN
Am J Trop Med Hyg, June 1, 2003; 68(6): 647 - 651.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Society of Tropical Medicine and Hygiene.