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Am. J. Trop. Med. Hyg., 60(3), 1999, pp. 487-492
Copyright © 1999 by The American Society of Tropical Medicine and Hygiene

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American Journal of Tropical Medicine and Hygiene, Vol 60, Issue 3, 487-492
Copyright © 1999 by American Society of Tropical Medicine and Hygiene

Research Articles


Blockage of skin invasion by schistosome cercariae by serine protease inhibitors

KC Lim, E Sun, M Bahgat, D Bucks, R Guy, RS Hinz, C Cullander, and JH McKerrow

Invasion of skin by schistosome cercariae is facilitated by a serine protease secreted from the acetabular cells of cercariae in response to skin lipid. Specific inhibitors of the protease, when applied to human skin in formulations designed to retain the inhibitor on and in the upper stratum corneum layers, block cercarial invasion of human skin. Both peptide-based, irreversible inhibitors and non-peptide, reversible inhibitors block cercarial invasion when applied in a propylene glycol:isopropyl alcohol (3:1) formulation in vitro. Arrest of cercarial invasion could be achieved even after immersion of treated skin in water for 2 hr. Peptide-based irreversible inhibitors in the presence of three different Topicare Delivery Compounds optimized arrest of cercarial invasion. The three Topicare Delivery Compounds applied alone prevented 80-100% of cercarial invasion. With inclusion of the inhibitor, there was 97-100% inhibition in vitro. The optimal formulation with inhibitor was then applied to the tails of BALB/c mice, and the mice were exposed to 120 cercariae by tail immersion. With the carrier lotion alone, there was a 50% reduction in worm burden and a 70% reduction in egg burden. When inhibitor was included, an 80% reduction in worm burden and a 92% reduction in egg burden was observed.


This article has been cited by other articles:


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 Mol. Cell. ProteomicsHome page
G. M. Knudsen, K. F. Medzihradszky, K.-C. Lim, E. Hansell, and J. H. McKerrow
Proteomic Analysis of Schistosoma mansoni Cercarial Secretions
Mol. Cell. Proteomics, December 1, 2005; 4(12): 1862 - 1875.
[Abstract] [Full Text] [PDF]

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 J. Biol. Chem.Home page
J. P. Salter, Y. Choe, H. Albrecht, C. Franklin, K.-C. Lim, C. S. Craik, and J. H. McKerrow
Cercarial Elastase Is Encoded by a Functionally Conserved Gene Family across Multiple Species of Schistosomes
J. Biol. Chem., June 28, 2002; 277(27): 24618 - 24624.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. P. Salter, K.-C. Lim, E. Hansell, I. Hsieh, and J. H. McKerrow
Schistosome Invasion of Human Skin and Degradation of Dermal Elastin Are Mediated by a Single Serine Protease
J. Biol. Chem., December 1, 2000; 275(49): 38667 - 38673.
[Abstract] [Full Text] [PDF]


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Copyright © 1999 by the American Society of Tropical Medicine and Hygiene.