AJTMH ASTMH Job Mart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 60(2), 1999, pp. 188-192
Copyright © 1999 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ito, A
Right arrow Articles by Pawlowski, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ito, A
Right arrow Articles by Pawlowski, Z.
Related Collections
Right arrow Diagnosis
Right arrow Echinococcosis
Right arrow Epidemiology
American Journal of Tropical Medicine and Hygiene, Vol 60, Issue 2, 188-192
Copyright © 1999 by American Society of Tropical Medicine and Hygiene

Research Articles


Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (EM18)

A Ito, L Ma, PM Schantz, B Gottstein, YH Liu, JJ Chai, SK Abdel-Hafez, N Altintas, DD Joshi, MW Lightowlers, and ZS Pawlowski

Echinococcus granulosus cyst fluid and E. multilocularis protoscolex extract were fractionated by a single step of preparative isoelectric focusing, resulting in an antigen B-rich fraction (8-kD) and an Em18-rich fraction, respectively. The usefulness of both fractions for differential serodiagnosis of cystic (CE) and alveolar (AE) echinococcosis was evaluated by a large-scale immunoblot analysis on a battery of 354 serum samples. These included 66 from AE patients originating from four different endemic areas, 173 from CE patients originating from seven different endemic areas, 71 from patients with other parasitic diseases, 15 from patients with hepatomas, and 29 from healthy individuals. In an immunoblot with the antigen B-rich fraction, 92% (158 of 173) of the CE sera as well as 79% (52 of 66) of the AE sera reacted with the 8-kD subunit. No cross-reactivity occurred with any sera from patients with cysticercosis, other parasitic diseases, or with hepatomas, or from healthy controls. In an immunoblot with the Em18-rich fraction, all but two sera from AE patients (64 of 66, 97%) recognized Em18, and only nine of 34 CE sera from China reacted with it. All other (139) CE sera from six other countries were negative as were all (115) other non-echinococcosis sera. These findings indicate that antigen B (8-kD) is not species-specific for E. granulosus but is genus-specific for Echinococcus, and that the Em18 antigen is a reliable serologic marker for species-specific differentiation of AE from CE.


This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
Y. R. YANG, M. ELLIS, T. SUN, Z. LI, X. LIU, D. A. VUITTON, B. BARTHOLOMOT, P. GIRAUDOUX, P. S. CRAIG, B. BOUFANA, et al.
UNIQUE FAMILY CLUSTERING OF HUMAN ECHINOCOCCOSIS CASES IN A CHINESE COMMUNITY.
Am J Trop Med Hyg, March 1, 2006; 74(3): 487 - 494.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
W. Mamuti, H. Yamasaki, Y. Sako, M. Nakao, N. Xiao, K. Nakaya, N. Sato, D. A. Vuitton, R. Piarroux, M. W. Lightowlers, et al.
Molecular Cloning, Expression, and Serological Evaluation of an 8-Kilodalton Subunit of Antigen B from Echinococcus multilocularis
J. Clin. Microbiol., March 1, 2004; 42(3): 1082 - 1088.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
R. Morar and C. Feldman
Pulmonary echinococcosis
Eur. Respir. J., June 1, 2003; 21(6): 1069 - 1077.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
W. Zhang, J. Li, and D. P. McManus
Concepts in Immunology and Diagnosis of Hydatid Disease
Clin. Microbiol. Rev., January 1, 2003; 16(1): 18 - 36.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
A. Ito, N. Xiao, M. Liance, M. O. Sato, Y. Sako, W. Mamuti, Y. Ishikawa, M. Nakao, H. Yamasaki, K. Nakaya, et al.
Evaluation of an Enzyme-Linked Immunosorbent Assay (ELISA) with Affinity-Purified Em18 and an ELISA with Recombinant Em18 for Differential Diagnosis of Alveolar Echinococcosis: Results of a Blind Test
J. Clin. Microbiol., November 1, 2002; 40(11): 4161 - 4165.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
Y. Sako, M. Nakao, K. Nakaya, H. Yamasaki, B. Gottstein, M. W. Lightowers, P. M. Schantz, and A. Ito
Alveolar Echinococcosis: Characterization of Diagnostic Antigen Em18 and Serological Evaluation of Recombinant Em18
J. Clin. Microbiol., August 1, 2002; 40(8): 2760 - 2765.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
W. Mamuti, H. Yamasaki, Y. Sako, K. Nakaya, M. Nakao, M. W. Lightowlers, and A. Ito
Usefulness of Hydatid Cyst Fluid of Echinococcus granulosus Developed in Mice with Secondary Infection for Serodiagnosis of Cystic Echinococcosis in Humans
Clin. Vaccine Immunol., May 1, 2002; 9(3): 573 - 576.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
Y. Sako, M. Nakao, T. Ikejima, X. Z. Piao, K. Nakaya, and A. Ito
Molecular Characterization and Diagnostic Value of Taenia solium Low-Molecular-Weight Antigen Genes
J. Clin. Microbiol., December 1, 2000; 38(12): 4439 - 4444.
[Abstract] [Full Text]

Home page
 J. Clin. Microbiol.Home page
M. Liance, V. Janin, S. Bresson-Hadni, D.-A. Vuitton, R. Houin, and R. Piarroux
Immunodiagnosis of Echinococcus Infections: Confirmatory Testing and Species Differentiation by a New Commercial Western Blot
J. Clin. Microbiol., October 1, 2000; 38(10): 3718 - 3721.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Society of Tropical Medicine and Hygiene.