AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
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Am. J. Trop. Med. Hyg., 60(1), 1999, pp. 124-128
Copyright © 1999 by The American Society of Tropical Medicine and Hygiene

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American Journal of Tropical Medicine and Hygiene, Vol 60, Issue 1, 124-128
Copyright © 1999 by American Society of Tropical Medicine and Hygiene

Research Articles


Pool screen polymerase chain reaction for estimating the prevalence of Onchocerca volvulus infection in Simulium damnosum sensu lato: results of a field trial in an area subject to successful vector control

L Yameogo, L Toe, JM Hougard, BA Boatin, and TR Unnasch

Detection of infective parasites in the vector population can be an early indicator of recrudescence in areas freed of new cases of onchocerciasis. However, dissection of vector black flies is inefficient in areas subject to effective control. Recently, a polymerase chain reaction (PCR)-based assay has been used to detect a single Onchocerca volvulus-infected black fly in pools containing large numbers of uninfected flies. This method had not been validated on wild-caught black flies in an area subject to effective vector control. Here, we report a method of restricting the pool screen PCR assay to infectious parasites and the results of a field test in an area subject to long-term vector control. The prevalence of infection determined by dissection did not differ from that determined by pool screen PCR. The results suggest that the PCR assay may be a useful tool for epidemiologic surveillance for 0. volvulus infection.


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C. R. KATHOLI and T. R. UNNASCH
Important experimental parameters for determining infection rates in arthropod vectors using pool screening approaches.
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M. A. RODRIGUEZ-PEREZ, B. G. LILLEY, A. DOMINGUEZ-VAZQUEZ, R. SEGURA-ARENAS, C. LIZARAZO-ORTEGA, A. MENDOZA-HERRERA, F. REYES-VILLANUEVA, and T. R. UNNASCH
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Am J Trop Med Hyg, January 1, 2004; 70(1): 38 - 45.
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A. G. GUEVARA, J. C. VIEIRA, B. G. LILLEY, A. LOPEZ, N. VIEIRA, J. RUMBEA, R. COLLINS, C. R. KATHOLI, and T. R. UNNASCH
ENTOMOLOGICAL EVALUATION BY POOL SCREEN POLYMERASE CHAIN REACTION OF ONCHOCERCA VOLVULUS TRANSMISSION IN ECUADOR FOLLOWING MASS MECTIZANTM DISTRIBUTION
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Copyright © 1999 by the American Society of Tropical Medicine and Hygiene.