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Am. J. Trop. Med. Hyg., 59(5), 1998, pp. 757-762
Copyright © 1998 by The American Society of Tropical Medicine and Hygiene

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American Journal of Tropical Medicine and Hygiene, Vol 59, Issue 5, 757-762
Copyright © 1998 by American Society of Tropical Medicine and Hygiene

Research Articles


Evaluation of an IgM immunoblot kit for dengue diagnosis

G Kuno, CB Cropp, J Wong-Lee, and DJ Gubler

A commercial IgM immunoblot kit was evaluated for dengue diagnosis with a panel of serum specimens collected from patients in a dengue endemic area. The kit is not recommended for use in its present form because of its undesirable rate of false-positive results. However, by substituting internal controls with the reference positive and negative controls that are more representative of those seen in endemic areas and by modifying the positive and negative scoring criteria, sensitivity and specificity of 80.3% and 94.5%, respectively, were obtained. These results are comparable with those obtained with the IgM ELISA on specimens, most of which were obtained from outpatient health care facilities. With further technical modifications, inclusion of a visual guide to ensure scoring standardization, and a more complete elaboration of the limitations of the test, wide application of the kit in diagnostic laboratories should be possible.


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Copyright © 1998 by the American Society of Tropical Medicine and Hygiene.