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Am. J. Trop. Med. Hyg., 59(3), 1998, pp. 347-351
Copyright © 1998 by The American Society of Tropical Medicine and Hygiene

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American Journal of Tropical Medicine and Hygiene, Vol 59, Issue 3, 347-351
Copyright © 1998 by American Society of Tropical Medicine and Hygiene

Research Articles


Development of monoclonal antibodies specifically recognizing the cyst stage of Entamoeba histolytica

B Walderich, GD Burchard, J Knobloch, and L Muller

Protozoan cysts were isolated according to a two-step sucrose gradient procedure. Pure cysts of Entamoeba histolytica, in fixed and native states, were injected into BALB/c mice intraperitoneally for immunization. The spleens of these animals were used for fusion with AG8 mouse myeloma cells. Hybridomas were obtained and tested for the recognition of E. histolytica, E. dispar, E. coli, E. hartmanni, Endolimax nana, Jodamoeba biitschlii, and Giardia lamblia. Three monoclonal antibodies were identified that reacted only with cysts and trophozoites of E. histolytica. These can be used for differentiation and identification of E. histolytica in feces.


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M. Frisardi, S. K. Ghosh, J. Field, K. Van Dellen, R. Rogers, P. Robbins, and J. Samuelson
The Most Abundant Glycoprotein of Amebic Cyst Walls (Jacob) Is a Lectin with Five Cys-Rich, Chitin-Binding Domains
Infect. Immun., July 1, 2000; 68(7): 4217 - 4224.
[Abstract] [Full Text] [PDF]


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Copyright © 1998 by the American Society of Tropical Medicine and Hygiene.