AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 59(2), 1998, pp. 291-294
Copyright © 1998 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ito, A
Right arrow Articles by Schantz, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ito, A
Right arrow Articles by Schantz, P.
Right arrowPubmed/NCBI databases
Medline Plus Health Information
*Neurologic Diseases
Related Collections
Right arrow Taeniasis
Right arrow Cestode Infections
Right arrow Cysticercosis
Right arrow Neurocysticercosis
American Journal of Tropical Medicine and Hygiene, Vol 59, Issue 2, 291-294
Copyright © 1998 by American Society of Tropical Medicine and Hygiene

Research Articles


Novel antigens for neurocysticercosis: simple method for preparation and evaluation for serodiagnosis

A Ito, A Plancarte, L Ma, Y Kong, A Flisser, SY Cho, YH Liu, S Kamhawi, MW Lightowlers, and PM Schantz

Neurocysticercosis (NCC), which is caused by infection with the larval stage of the pork tapeworm (Taenia solium), is now recognized as a major cause of neurologic diseases in countries where the infection is endemic. Migration of persons from these countries is resulting in diagnosis and local transmission in nonendemic countries at increasing rates. In the present study, immunoblotting and an ELISA were carried out using antigens of T. solium cysticerci fractionated by isoelectric focusing and serum samples from patients with NCC, alveolar (AE) or cystic echinococcosis (CE), and other diseases. Immunoblot analysis revealed antigens fractionated by isoelectric focusing (pH 9.2-9.6) either from cyst fluid of T. solium cysticerci or from intact cysts had unique components (glycoproteins) highly specific and sensitive for detection of NCC exclusively. All confirmed NCC serum samples (53 of 53) recognized at least three major bands of 10-26-kD of fractions with pH 9.2-9.6 from either intact cysts or cyst fluid. These bands were not recognized by sera from patients with other parasitic diseases including AE (0 of 34), CE (0 of 36), or other heterologous parasitoses (0 of 77), patients with hepatoma (0 of 19) or sarcoidosis (0 of 11), or sera from healthy controls (0 of 29). The ELISA using the antigens showed the same sensitivity and specificity for differentiation of NCC (53 of 53) from other diseases (0 of 107) or healthy individuals (0 of 29). Both immunoblotting and the ELISA using the fractionated antigens readily differentiated all NCC from AE or CE in a blind test of 29 serum samples of persons with NCC, CE, and AE. Antigens fractionated from cyst fluid of T. solium cysticerci by a simple, single-step isoelectric focusing (pH 9.2-9.6) are highly specific and sensitive for differential serodiagnosis of NCC in immunoblotting and/or an ELISA.


This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
N. Myadagsuren, A. Davaajav, T. Wandra, T. Sandar, P. Ichinkhorloo, H. Yamasaki, Y. Sako, M. Nakao, M. O. Sato, K. Nakaya, et al.
Taeniasis in Mongolia, 2002 2006
Am J Trop Med Hyg, August 1, 2007; 77(2): 342 - 346.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
M. O. SATO, T. V. CAVALCANTE, Y. SAKO, M. NAKAO, H. YAMASAKI, A. P. YATSUDA, K. NAKAYA, and A. ITO
EVIDENCE AND POTENTIAL FOR TRANSMISSION OF HUMAN AND SWINE TAENIA SOLIUM CYSTICERCOSIS IN THE PIRACURUCA REGION, PIAUI, BRAZIL
Am J Trop Med Hyg, November 1, 2006; 75(5): 933 - 935.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
H. Yamasaki, S. Matsunaga, K. Yamamura, C.-C. Chang, S. Kawamura, Y. Sako, M. Nakao, K. Nakaya, and A. Ito
Solitary Neurocysticercosis Case Caused by Asian Genotype of Taenia solium Confirmed by Mitochondrial DNA Analysis
J. Clin. Microbiol., August 1, 2004; 42(8): 3891 - 3893.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
W. Mamuti, H. Yamasaki, Y. Sako, M. Nakao, N. Xiao, K. Nakaya, N. Sato, D. A. Vuitton, R. Piarroux, M. W. Lightowlers, et al.
Molecular Cloning, Expression, and Serological Evaluation of an 8-Kilodalton Subunit of Antigen B from Echinococcus multilocularis
J. Clin. Microbiol., March 1, 2004; 42(3): 1082 - 1088.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
Y. Sako, M. Nakao, K. Nakaya, H. Yamasaki, B. Gottstein, M. W. Lightowers, P. M. Schantz, and A. Ito
Alveolar Echinococcosis: Characterization of Diagnostic Antigen Em18 and Serological Evaluation of Recombinant Em18
J. Clin. Microbiol., August 1, 2002; 40(8): 2760 - 2765.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
A. X. Pardini, A. J. Vaz, L. D. R. Machado, and J. A. Livramento
Cysticercus Antigens in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis
J. Clin. Microbiol., September 1, 2001; 39(9): 3368 - 3372.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
Y. Sako, M. Nakao, T. Ikejima, X. Z. Piao, K. Nakaya, and A. Ito
Molecular Characterization and Diagnostic Value of Taenia solium Low-Molecular-Weight Antigen Genes
J. Clin. Microbiol., December 1, 2000; 38(12): 4439 - 4444.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American Society of Tropical Medicine and Hygiene.