| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
This report describes the first detailed analysis of the replication, persistence, and excretion of a North American hantavirus in its natural rodent reservoir. Black Creek Canal virus was isolated from Sigmodon hispidus (cotton rat) shortly after the identification of a hantavirus pulmonary syndrome (HPS) case occurring in southern Florida. Six-week-old male cotton rats were inoculated subcutaneously with 1,000 tissue culture infectious doses. Viral complementary RNA (vcRNA) was quantified as a means of determining the site(s) of viral activity (transcription and replication). In the first few weeks post inoculation (pi), vcRNA was detectable in every tissue examined except blood. The quantities of vcRNA decreased over time, and by five months pi it could be detected only in the brain. In addition to using a quantitative polymerase chain reaction (QPCR) as a means of measuring viral replication/transcription, attempts were made to reisolate virus from all tissue samples taken. Virus could be isolated from every solid tissue examined, and the titers appeared to decrease over time, similar to the QPCR results. However, in contrast to the QPCR results, infectious virus was still routinely detectable at low levels in adrenal gland, liver, kidney, and testicle 150 days pi. Although results of testing for vcRNA in the blood were uniformly negative, infectious virus was detected at one week pi, reached highest titers at two weeks, and decreased dramatically by three weeks. After three weeks pi, infectious virus could only be detected sporadically in blood. Virus was isolated from urine collected during the first 70 days pi and throughout the entire study period in feces and wet bedding. These data indicate that the viral infection can be separated into an acute phase associated with high virus titers, and a chronic or persistent phase associated with lower virus titers and continued shedding of virus in excreta.
This article has been cited by other articles:
|
|
 |
|
  J. R. Levine, C. L. Fritz, and M. G. Novak Occupational Risk of Exposure to Rodent-borne Hantavirus at US Forest Service Facilities in California Am J Trop Med Hyg, February 1, 2008; 78(2): 352 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Douglass, C. H. Calisher, K. D. Wagoner, and J. N. Mills SIN NOMBRE VIRUS INFECTION OF DEER MICE IN MONTANA: CHARACTERISTICS OF NEWLY INFECTED MICE, INCIDENCE, AND TEMPORAL PATTERN OF INFECTION J. Wildl. Dis., January 1, 2007; 43(1): 12 - 22. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. SAFRONETZ, R. LINDSAY, B. HJELLE, R. A. MEDINA, K. MIROWSKY-GARCIA, and M. A. DREBOT USE OF IgG AVIDITY TO INDIRECTLY MONITOR EPIZOOTIC TRANSMISSION OF SIN NOMBRE VIRUS IN DEER MICE (PEROMYSCUS MANICULATUS) Am J Trop Med Hyg, December 1, 2006; 75(6): 1135 - 1139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. R. Kallio, J. Klingstrom, E. Gustafsson, T. Manni, A. Vaheri, H. Henttonen, O. Vapalahti, and A. Lundkvist Prolonged survival of Puumala hantavirus outside the host: evidence for indirect transmission via the environment. J. Gen. Virol., August 1, 2006; 87(Pt 8): 2127 - 2134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. KEMPF, C. D. BLAIR, and B. J. BEATY QUANTITATIVE ANALYSIS OF LA CROSSE VIRUS TRANSCRIPTION AND REPLICATION IN CELL CULTURES AND MOSQUITOES Am J Trop Med Hyg, February 1, 2006; 74(2): 224 - 232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. E. MCINTYRE, Y.-K. CHU, R. D. OWEN, A. ABUZEINEH, N. DE LA SANCHA, C. W. DICK, T. HOLSOMBACK, R. A. NISBETT, and C. JONSSON A LONGITUDINAL STUDY OF BAYOU VIRUS, HOSTS, AND HABITAT Am J Trop Med Hyg, December 1, 2005; 73(6): 1043 - 1049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Kuenzi, R. J. Douglass, C. W. Bond, C. H. Calisher, and J. N. Mills LONG-TERM DYNAMICS OF SIN NOMBRE VIRAL RNA AND ANTIBODY IN DEER MICE IN MONTANA J. Wildl. Dis., July 1, 2005; 41(3): 473 - 481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Padula, R. Figueroa, M. Navarrete, E. Pizarro, R. Cadiz, C. Bellomo, C. Jofre, L. Zaror, E. Rodriguez, and R. Murua Transmission Study of Andes Hantavirus Infection in Wild Sigmodontine Rodents J. Virol., November 1, 2004; 78(21): 11972 - 11979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Botten, K. Mirowsky, D. Kusewitt, C. Ye, K. Gottlieb, J. Prescott, and B. Hjelle Persistent Sin Nombre Virus Infection in the Deer Mouse (Peromyscus maniculatus) Model: Sites of Replication and Strand-Specific Expression J. Virol., December 20, 2002; 77(2): 1540 - 1550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Botten, K. Mirowsky, C. Ye, K. Gottlieb, M. Saavedra, L. Ponce, and B. Hjelle Shedding and Intracage Transmission of Sin Nombre Hantavirus in the Deer Mouse (Peromyscus maniculatus) Model J. Virol., June 27, 2002; 76(15): 7587 - 7594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ebihara, K. Yoshimatsu, M. Ogino, K. Araki, Y. Ami, H. Kariwa, I. Takashima, D. Li, and J. Arikawa Pathogenicity of Hantaan Virus in Newborn Mice: Genetic Reassortant Study Demonstrating that a Single Amino Acid Change in Glycoprotein G1 Is Related to Virulence J. Virol., October 1, 2000; 74(19): 9245 - 9255. [Abstract] [Full Text]
|
|
|
|
|
|
M. K. Borucki, J. D. Boone, J. E. Rowe, M. C. Bohlman, E. A. Kuhn, R. DeBaca, and S. C. St. Jeor Role of Maternal Antibody in Natural Infection of Peromyscus maniculatus with Sin Nombre Virus J. Virol., March 1, 2000; 74(5): 2426 - 2429. [Abstract] [Full Text]
|
|
|
|
|
|
B. J. Meyer and C. Schmaljohn Accumulation of Terminally Deleted RNAs May Play a Role in Seoul Virus Persistence J. Virol., February 1, 2000; 74(3): 1321 - 1331. [Abstract] [Full Text]
|
|
|
|
|
|
R. Feuer, J. D. Boone, D. Netski, S. P. Morzunov, and S. C. St. Jeor Temporal and Spatial Analysis of Sin Nombre Virus Quasispecies in Naturally Infected Rodents J. Virol., November 1, 1999; 73(11): 9544 - 9554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Netski, B. H. Thran, and S. C. St. Jeor Sin Nombre Virus Pathogenesis in Peromyscus maniculatus J. Virol., January 1, 1999; 73(1): 585 - 591. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Botten, K. Mirowsky, D. Kusewitt, M. Bharadwaj, J. Yee, R. Ricci, R. M. Feddersen, and B. Hjelle Experimental infection model for Sin Nombre hantavirus in the deer mouse (Peromyscus maniculatus) PNAS, September 12, 2000; 97(19): 10578 - 10583. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
