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Evaluation of the Kato-Katz Thick Smear and Formal Ether Sedimentation Techniques for Quantitative Diagnosis of Schistosoma mansoni Infection

The present study compared the Kato-Katz thick smear and formol ether sedimentation techniques in the diagnosis of Schistosoma mansoni infections. A stool specimen was collected from 915 individuals representing a high prevalence community (63.3%) and from 471 individuals representing a relatively low prevalence village (40%). The overall sensitivity of a single Kato-Katz smear was 70.8%, and it increased with each additional slide to reach 91.7% on examining four smears. However, the sensitivity was 83.3% when using the formol ether sedimentation technique. In terms of quantitative analysis, the geometric mean egg count was 94 eggs per gram (epg) of stool by two Kato-Katz smears, and 43 epg by the sedimentation technique. This means that more than 50% of eggs were missed when using the sedimentation technique, a fact that should be taken into consideration when relating infection level with morbidity.

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				D. GOODMAN, H. J. HAJI, Q. D. BICKLE, R. J. STOLTZFUS, J. M. TIELSCH, M. RAMSAN, L. SAVIOLI, and M. ALBONICO A COMPARISON OF METHODS FOR DETECTING THE EGGS OF ASCARIS, TRICHURIS, AND HOOKWORM IN INFANT STOOL, AND THE EPIDEMIOLOGY OF INFECTION IN ZANZIBARI INFANTS Am J Trop Med Hyg, April 1, 2007; 76(4): 725 - 731. [Abstract] [Full Text] [PDF]