AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 57(6), 1997, pp. 683-686
Copyright © 1997 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ortega, Y. R.
Right arrow Articles by Sterling, C. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ortega, Y. R.
Right arrow Articles by Sterling, C. R.

Isolation of Cryptosporidium parvum and Cyclospora cayetanensis from Vegetables Collected in Markets of an Endemic Region in Peru

Ynes R. Ortega, Concepcion R. Roxas, Robert H. Gilman, Norma J. Miller, Lilia Cabrera, Carmen Taquiri AND Charles R. Sterling
Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona; Asociacion Benefica Proyectos en Informatica Salud, Medicina y Agricultura, Lima, Peru; Departamento de Patologia, Universidad Peruana Cayetano Heredia, Lima, Peru; Department of International Health, The Johns Hopkins University, Baltimore, Maryland

Cryptosporidium parvum and Cyclospora cayetanensis are protozoan pathogens that cause prolonged diarrhea in both immunocompetent and immunocompromised hosts. Cryptosporidium parvum can be transmitted via the fecal-oral route, while the exact mechanisms of transmission of Cyclospora cayetanensis have not been fully determined. Humans appear to be the sole host for the latter and a distinct seasonality has been observed in endemic areas around the world. Samples of vegetables were collected at several small markets in a periurban slum in Peru during the seasons of high and low incidence. The vegetables were washed, the supernatants were collected and centrifuged, and the pellets were resuspended in a solution of 2.5% potassium dichromate. Pellets were examined using direct microscopic observation, acid-fast staining, and immunofluorescent assays for C. parvum and Cyclospora cayetanensis oocysts. Samples were collected during three time periods: the season of low incidence, the beginning of the season of high incidence, and end of the season of high incidence. Of the total vegetables examined, 14.5% contained C. parvum oocysts and 1.8% had Cyclospora oocysts. Thus, market vegetables may provide a route by which Cryptosporidium and Cyclospora can be transmitted. Our study also suggests that washing vegetables does not completely remove Cryptosporidium and Cyclospora oocysts.




This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
C. D. Frazar and P. A. Orlandi
Evaluation of Two DNA Template Preparation Methods for Post-Immunomagnetic Separation Detection of Cryptosporidium parvum in Foods and Beverages by PCR
Appl. Envir. Microbiol., November 15, 2007; 73(22): 7474 - 7476.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
P. A. TORRES-SLIMMING, C. C. MUNDACA, M. MORAN, J. QUISPE, O. COLINA, D. J. BACON, A. G. LESCANO, R. H. GILMAN, and D. L. BLAZES
Outbreak of cyclosporiasis at a naval base in lima, peru.
Am J Trop Med Hyg, September 1, 2006; 75(3): 546 - 548.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the American Society of Tropical Medicine and Hygiene.