AJTMH ASTMH Job Mart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 57(4), 1997, pp. 495-500
Copyright © 1997 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cheng, Q.
Right arrow Articles by Saul, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, Q.
Right arrow Articles by Saul, A.

Measurement of Plasmodium falciparum Growth Rates in Vivo: A Test of Malaria Vaccines

Qin Cheng, Gregor Lawrence, Carol Reed, Anthony Stowers, Lisa Ranford-Cartwright, Alison Creasey, Richard Carter AND Allan Saul
Malaria and Arbovirus Unit and The Cooperative Research Centre for Vaccine Technology, Queensland Institute of Medical Research, Brisbane, Australia; Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh, United Kingdom

Several prototype vaccines against the asexual blood stage of malaria are undergoing preclinical and phase I testing. Although these vaccines have been chosen for their ability to elicit an anti-parasite response, no practical and sensitive clinical trial procedure has been available for measuring their impact on parasite growth. We describe a system that allows parasite growth rates to be measured in volunteers through the incubation period. Two necessary elements of this system are developed: suitable blood-stage Plasmodium falciparum inocula, and a highly sensitive and quantitative assay to measure parasite growth during the incubation period. We infected five nonimmune volunteers with an inoculum as small as 300 parasites and demonstrated that the resultant in vivo asexual parasite growth rates were reproducible at 12–15-fold per cycle. The system allowed the infection to be followed for eight days before treatment without symptoms developing. These findings suggest that it is feasible to directly measure the anti-parasite efficacy of a prototype malaria vaccine in human volunteers without subjecting them to the risk of disease.




This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
M. M. Nyunt, C. W. Hendrix, R. P. Bakshi, N. Kumar, and T. A. Shapiro
Phase I/II Evaluation of the Prophylactic Antimalarial Activity of Pafuramidine in Healthy Volunteers Challenged with Plasmodium falciparum Sporozoites
Am J Trop Med Hyg, April 1, 2009; 80(4): 528 - 535.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
F. Sanderson, L. Andrews, A. D. Douglas, A. Hunt-Cooke, P. Bejon, and A. V. S. Hill
Blood-stage Challenge for Malaria Vaccine Efficacy Trials: A Pilot Study with Discussion of Safety and Potential Value
Am J Trop Med Hyg, June 1, 2008; 78(6): 878 - 883.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. R. Krause, M. L. Gatton, S. Frankland, D. P. Eisen, M. F. Good, L. Tilley, and Q. Cheng
Characterization of the Antibody Response against Plasmodium falciparum Erythrocyte Membrane Protein 1 in Human Volunteers
Infect. Immun., December 1, 2007; 75(12): 5967 - 5973.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
M. P. Dal-Bianco, K. B. Koster, U. D. Kombila, J. F. J. Kun, M. P. Grobusch, G. M. Ngoma, P. B. Matsiegui, C. Supan, C. L. Ospina Salazar, M. A. Missinou, et al.
High Prevalence of Asymptomatic Plasmodium falciparum Infection in Gabonese Adults
Am J Trop Med Hyg, November 1, 2007; 77(5): 939 - 942.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
M. L. GATTON, J. M. PETERS, K. GRESTY, E. V. FOWLER, N. CHEN, and Q. CHENG
DETECTION SENSITIVITY AND QUANTITATION OF PLASMODIUM FALCIPARUM VAR GENE TRANSCRIPTS BY REAL-TIME RT-PCR IN COMPARISON WITH CONVENTIONAL RT-PCR.
Am J Trop Med Hyg, August 1, 2006; 75(2): 212 - 218.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
A.-M. DEANS, K. E. LYKE, M. A. THERA, C. V. PLOWE, A. KONE, O. K. DOUMBO, O. KAI, K. MARSH, M. J. MACKINNON, A. RAZA, et al.
LOW MULTIPLICATION RATES OF AFRICAN PLASMODIUM FALCIPARUM ISOLATES AND LACK OF ASSOCIATION OF MULTIPLICATION RATE AND RED BLOOD CELL SELECTIVITY WITH MALARIA VIRULENCE
Am J Trop Med Hyg, April 1, 2006; 74(4): 554 - 563.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Peters, E. Fowler, M. Gatton, N. Chen, A. Saul, and Q. Cheng
High diversity and rapid changeover of expressed var genes during the acute phase of Plasmodium falciparum infections in human volunteers
PNAS, August 6, 2002; 99(16): 10689 - 10694.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the American Society of Tropical Medicine and Hygiene.