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Am. J. Trop. Med. Hyg., 54(6), 1996, pp. 629-631
Copyright © 1996 by The American Society of Tropical Medicine and Hygiene

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Short Report: Simultaneous Detection by Polymerase Chain Reaction of Mosquito Species and Plasmodium falciparum Infection in Anopheles gambiae Sensu Lato

Madama Bouare, Djibril Sangare, Magaran Bagayoko, Abdoulaye Toure, Yeya T. Toure AND Kenneth D. Vernick
Departement d'Epidemiologie des Affectations Parasitaires, Ecole Nationale de Medecine et de Pharmacie, Bamako, Mali; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

Total RNA purified from Anopheles gambiae mosquitoes can be used for detection of both 1) infection by Plasmodium falciparum using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay specific for P. falciparum ribosomal RNA (rRNA) of sporogonic stage parasites, and 2) mosquito species using a PCR assay that distinguishes members of the Anopheles gambiae complex.






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