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Am. J. Trop. Med. Hyg., 53(1), 1995, pp. 43-46
Copyright © 1995 by The American Society of Tropical Medicine and Hygiene

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Effectiveness of a Dot-Blot Immunoassay of Anti-Rickettsia tsutsugamushi Antibodies for Serologic Analysis of Scrub Typhus

J. R. Weddle, T.-C. Chan, K. Thompson, H. Paxton, D. J. Kelly, G. Dasch AND D. Strickman
Viral and Rickettsial Diseases Program, Naval Medical Research Institute, Bethesda, Maryland; Integrated Diagnostics, Inc., Baltimore, Maryland; Department of Clinical Investigations, Walter Reed Army Medical Center, Washington, District of Columbia; Department of Rickettsial Diseases, Walter Reed Army Institute of Research, Washington, District of Columbia

We compared a commercially available dot-blot immunoassay system with the indirect immunofluorescence assay (IFA) in tests of known negative and known positive sera from scrub typhus cases. Using a panel of 100 sera from patients with various rickettsial and nonrickettsial infections, we observed that the IFA was 99% specific and the dipstick assay was 98% specific. In tests of 91 sera (30 negative and 61 positive for scrub typhus antibodies) from a study of febrile patients in Malaysia, using the standard of an IFA titer < 1:64 as negative, an IFA titer > 1:128 as positive, and an IFA titer = 1:64 as either positive or negative (supported by clinical records), dipsticks were 83% specific and 90% sensitive. The quantitative correlation of the dipsticks to IFA titers was confirmed by significant differences in geometric means of inverse IFA titers corresponding to the number of positive dipstick spots (no dots = 8.5, one dot = 43.3, two dots = 206.7, and three dots = 676.9). The assay would enable physicians and public health workers who deal with patients to quickly diagnose and appropriately treat most cases of the disease, especially in areas of high prevalence where the proportion of false-positive results to true-positive results would be low.




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M. CAO, H. GUO, T. TANG, C. WANG, X. LI, X. PAN, Z. JIN, and J. TANG
PREPARATION OF RECOMBINANT ANTIGEN OF O. TSUTSUGAMUSHI PTAN STRAIN AND DEVELOPMENT OF RAPID DIAGNOSTIC REAGENT FOR SCRUB TYPHUS
Am J Trop Med Hyg, March 1, 2007; 76(3): 553 - 558.
[Abstract] [Full Text] [PDF]


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Copyright © 1995 by the American Society of Tropical Medicine and Hygiene.