AJTMH ASTMH MEMBERSHIP INFORMATION: astmh@astmh.org
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 51(5), 1994, pp. 603-612
Copyright © 1994 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hoffman, S. L.
Right arrow Articles by Jones, T. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hoffman, S. L.
Right arrow Articles by Jones, T. R.

Safety, Immunogenicity, and Efficacy of a Malaria Sporozoite Vaccine Administered with Monophosphoryl Lipid A, Cell Wall Skeleton of Mycobacteria, and Squalane as Adjuvant

Stephen L. Hoffman, Robert Edelman, Joe P. Bryan, Imogene Schneider, Jonathan Davis, Martha Sedegah, Daniel Gordon, Preston Church, Mitchell Gross, Carol Silverman, Michael Hollingdale, David Clyde, Marcelo Sztein, Genevieve Losonsky, Scott Paparello AND Trevor R. Jones
Malaria Program, Naval Medical Research Institute, Bethesda, Maryland; Center for Vaccine Development, University of Maryland, Baltimore, Maryland; Department of Preventive Medicine and Biometrics, Uniformed Services University of the Health Sciences, Bethesda, Maryland; Department of Entomology and Department of Immunology, Walter Reed Army Institute of Research, Washington, District of Columbia; Pan American Health Organization, Washington, District of Columbia; SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania; Biomedical Research Institute, Rockville, Maryland; Division of Infectious Diseases, National Naval Medical Center, Bethesda, Maryland

A Plasmodium falciparum circumsporozoite protein (PfCSP) recombinant fusion protein, R32NSI81, formulated with monophosphoryl lipid A, cell wall skeleton of mycobacteria, and squalane (DetoxTM) was administered to 12 volunteers. One volunteer had malaise and self-limited painful induration at the injection site after the second dose and declined further immunization. The other 11 volunteers tolerated the three doses of 1,230 µg of vaccine, but most complained of sore arms; in five cases the pain or malaise was severe enough to interfere with work or sleep. Two weeks after the third dose of vaccine, four of the 11 immunized volunteers had ≥ 14 µg/ml of antibodies to the repeat region of the PfCSP in their serum. Two of these four volunteers did not develop P. falciparum parasitemia when challenged by the bite of five mosquitoes carrying P. falciparum sporozoites. The seven volunteers with lower levels of antibodies and 11 of 11 controls developed parasitemia. These data are consistent with other studies, and indicate that vaccine-induced antibodies against the repeat region of PfCSP can prevent effective sporozoite infection of hepatocytes in humans. The challenge is to improve the immunogenicity of PfCSP-based vaccines, and to develop methods for including PfCSP peptides as components of multitarget malaria vaccines.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
R. Chattopadhyay, D. Rathore, H. Fujioka, S. Kumar, P. de la Vega, D. Haynes, K. Moch, D. Fryauff, R. Wang, D. J. Carucci, et al.
PfSPATR, a Plasmodium falciparum Protein Containing an Altered Thrombospondin Type I Repeat Domain Is Expressed at Several Stages of the Parasite Life Cycle and Is the Target of Inhibitory Antibodies
J. Biol. Chem., July 3, 2003; 278(28): 25977 - 25981.
[Abstract] [Full Text] [PDF]

Home page
 Br Med BullHome page
P. Moingeon, C. de Taisne, and J. Almond
Delivery technologies for human vaccines
Br. Med. Bull., July 1, 2002; 62(1): 29 - 44.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the American Society of Tropical Medicine and Hygiene.