HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Feighny, R. Articles by Putnak, R. Search for Related Content PubMed PubMed Citation Articles by Feighny, R. Articles by Putnak, R.

Dengue Type-2 Virus Envelope Protein Made Using Recombinant Baculovirus Protects Mice against Virus Challenge

The gene coding for the envelope (E) glycoprotein of dengue-2 virus was cloned into baculovirus (Autographa californica nuclear polyhedrosis virus). The recombinant virus contained the entire E protein gene, preceded by 38 nucleotides from the end of the prematrix glycoprotein gene and followed by the first 83 nucleotides of nonstructural protein 1. When expressed in Spodoptera frugiperda (Sf9) cells, approximately 1 mg of recombinant E antigen was made per 10⁹ cells. This antigen reacted with polyclonal, antidengue type-2 antibody and a dengue type-2-specific, neutralizing monoclonal antibody. BALB/c mice immunized with the recombinant antigen produced only non-neutralizing antibody against dengue-2 virus, but were partially protected against morbidity and mortality after intracranial challenge with virulent dengue-2 virus.

^* Deceased.