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An Evaluation of the Standardized Direct Smear for Egg Counting in Parasitological Work¹

The standardized direct smear and the Stoll dilution egg counting methods were compared in 1,000 fecal samples. Of these 411 had shown A. lumbricoides, 951 T. trichiura, 449 N. americanus and 310 S. mansoni eggs.

The data obtained with the two methods were analyzed by the x² test and coefficient of correlation. In general the techniques compared favorably. The A. lumbricoides was represented by all degrees of intensity of infection and gave highly significant results. A majority of the infections with the other three helminths were light. It is believed that this led to the less significant results obtained with these species. It is concluded that the standardized smear is the technique of choice.

¹ The present work has been carried out with the partial support of the Department of the Army Contract No. DA-49-007-MD-24.